Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2629680..2630322 Replicon chromosome
Accession NZ_CP072691
Organism Bacillus thuringiensis strain IMBL-B9

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag J2N67_RS13445 Protein ID WP_000635965.1
Coordinates 2629680..2630030 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag J2N67_RS13450 Protein ID WP_000004570.1
Coordinates 2630035..2630322 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J2N67_RS13430 (J2N67_002688) 2626615..2627073 - 459 WP_000344239.1 SprT family protein -
J2N67_RS13435 2627269..2627385 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
J2N67_RS13440 (J2N67_002689) 2627444..2629612 - 2169 WP_000426236.1 Tex family protein -
J2N67_RS13445 (J2N67_002690) 2629680..2630030 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J2N67_RS13450 (J2N67_002691) 2630035..2630322 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
J2N67_RS13455 (J2N67_002692) 2630631..2631800 - 1170 WP_000390616.1 alanine racemase -
J2N67_RS13460 (J2N67_002693) 2631918..2632868 - 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
J2N67_RS13465 (J2N67_002694) 2633025..2633384 - 360 WP_000583417.1 holo-ACP synthase -
J2N67_RS13470 (J2N67_002695) 2633478..2634050 + 573 WP_000908522.1 rhomboid family intramembrane serine protease -
J2N67_RS13475 (J2N67_002696) 2634043..2635005 - 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T198334 WP_000635965.1 NZ_CP072691:c2630030-2629680 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T198334 NZ_CP096845:1747989-1748393 [Mycobacterium tuberculosis variant bovis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT198334 WP_000004570.1 NZ_CP072691:c2630322-2630035 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT198334 NZ_CP096845:1747780-1747983 [Mycobacterium tuberculosis variant bovis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References