Detailed information of TA system TA198333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 350074..351052
• Replicon: chromosome
• Accession: NZ_CP072691
• Organism: Bacillus thuringiensis strain IMBL-B9

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: J2N67_RS01555
• Protein ID: WP_000624977.1
• Coordinates: 350315..351052 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: J2N67_RS01550
• Protein ID: WP_000237818.1
• Coordinates: 350074..350202 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2N67_RS01520 (J2N67_000302)	345562..345813	+	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-
J2N67_RS01525 (J2N67_000303)	346053..346655	-	603	WP_000517260.1	hypothetical protein	-
J2N67_RS01530 (J2N67_000304)	346681..347490	-	810	WP_000678509.1	papain-like cysteine protease family protein	-
J2N67_RS01535 (J2N67_000305)	347736..349205	-	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
J2N67_RS01540 (J2N67_000306)	349417..349806	+	390	WP_000713866.1	YxeA family protein	-
J2N67_RS01545 (J2N67_000307)	349825..350001	-	177	WP_000852629.1	stage II sporulation protein SB	-
J2N67_RS01550 (J2N67_000308)	350074..350202	-	129	WP_000237818.1	hypothetical protein	Antitoxin
J2N67_RS01555 (J2N67_000309)	350315..351052	-	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J2N67_RS01560 (J2N67_000310)	351290..352027	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
J2N67_RS01565 (J2N67_000311)	352194..352676	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
J2N67_RS01570 (J2N67_000312)	352784..354469	+	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
J2N67_RS01575 (J2N67_000313)	354627..355409	-	783	WP_000381800.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T198333 WP_000624977.1 NZ_CP072691:c351052-350315 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T198333 NZ_CP096845:1680156-1680473 [Mycobacterium tuberculosis variant bovis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT198333 WP_000237818.1 NZ_CP072691:c350202-350074 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT198333 NZ_CP096845:1679857-1680159 [Mycobacterium tuberculosis variant bovis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5