Detailed information of TA system TA198324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2294813..2295451
• Replicon: chromosome
• Accession: NZ_CP072689
• Organism: Escherichia marmotae strain YF8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: J8I93_RS10740
• Protein ID: WP_000813795.1
• Coordinates: 2295275..2295451 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J8I93_RS10735
• Protein ID: WP_077627724.1
• Coordinates: 2294813..2295229 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I93_RS10715 (2289968)	2289968..2290909	-	942	WP_001516417.1	ABC transporter permease	-
J8I93_RS10720 (2290910)	2290910..2291923	-	1014	WP_282502716.1	ABC transporter ATP-binding protein	-
J8I93_RS10725 (2291941)	2291941..2293086	-	1146	WP_000047463.1	ABC transporter substrate-binding protein	-
J8I93_RS10730 (2293325)	2293325..2294734	-	1410	WP_061091001.1	PLP-dependent aminotransferase family protein	-
J8I93_RS10735 (2294813)	2294813..2295229	-	417	WP_077627724.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J8I93_RS10740 (2295275)	2295275..2295451	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J8I93_RS10745 (2295673)	2295673..2295903	+	231	WP_000494240.1	DUF2554 family protein	-
J8I93_RS10750 (2295997)	2295997..2297958	-	1962	WP_024191881.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J8I93_RS10755 (2298031)	2298031..2298567	-	537	WP_061091000.1	XRE family transcriptional regulator	-
J8I93_RS10760 (2298659)	2298659..2299831	+	1173	WP_024191882.1	benzoate/H(+) symporter BenE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T198324 WP_000813795.1 NZ_CP072689:c2295451-2295275 [Escherichia marmotae]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T198324 NZ_CP096845:839861-840289 [Mycobacterium tuberculosis variant bovis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTGGTGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15250.62 Da
• Isoelectric Point: 4.5908

>AT198324 WP_077627724.1 NZ_CP072689:c2295229-2294813 [Escherichia marmotae]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNNNDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT198324 NZ_CP096845:839580-839837 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1