Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 378842..379338 Replicon chromosome
Accession NZ_CP072689
Organism Escherichia marmotae strain YF8

Toxin (Protein)


Gene name ghoT Uniprot ID A0A7U9DCZ4
Locus tag J8I93_RS01715 Protein ID WP_001173345.1
Coordinates 379165..379338 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A7U9DAJ1
Locus tag J8I93_RS01710 Protein ID WP_001517605.1
Coordinates 378842..379138 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I93_RS01690 (375574) 375574..376293 - 720 WP_000611294.1 two-component system response regulator DcuR -
J8I93_RS01695 (376290) 376290..377921 - 1632 WP_282502912.1 sensor histidine kinase -
J8I93_RS01700 (378100) 378100..378330 + 231 WP_000371696.1 4Fe-4S mono-cluster protein YjdI -
J8I93_RS01705 (378342) 378342..378614 + 273 WP_000405652.1 GNAT family N-acetyltransferase -
J8I93_RS01710 (378842) 378842..379138 + 297 WP_001517605.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J8I93_RS01715 (379165) 379165..379338 + 174 WP_001173345.1 type V toxin-antitoxin system toxin GhoT Toxin
J8I93_RS01720 (379457) 379457..380974 - 1518 WP_001295074.1 lysine--tRNA ligase -
J8I93_RS01725 (381211) 381211..382668 - 1458 WP_001517606.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 378842..398908 20066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198308 WP_001173345.1 NZ_CP072689:379165-379338 [Escherichia marmotae]
MALFSKILIFYVIGVNISFVIIWFLSHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198308 NZ_CP096845:71512-71913 [Mycobacterium tuberculosis variant bovis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11568.18 Da        Isoelectric Point: 4.0994

>AT198308 WP_001517605.1 NZ_CP072689:378842-379138 [Escherichia marmotae]
MEGINRFKTYVVSFDYPSSYYSVFLRLRSLMYDMDFSSIVADEYGIPRQLNENAFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMIVDDYFRQFYK

Download         Length: 297 bp

>AT198308 NZ_CP096845:71280-71519 [Mycobacterium tuberculosis variant bovis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGTAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9DCZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAJ1

References