Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4586181..4586678 Replicon chromosome
Accession NZ_CP072688
Organism Escherichia coli strain P4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J8I92_RS22330 Protein ID WP_001173343.1
Coordinates 4586181..4586354 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag J8I92_RS22335 Protein ID WP_001312331.1
Coordinates 4586382..4586678 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I92_RS22320 (4582851) 4582851..4584308 + 1458 WP_000856828.1 dipeptide/tripeptide permease DtpC -
J8I92_RS22325 (4584545) 4584545..4586062 + 1518 WP_000003805.1 lysine--tRNA ligase -
J8I92_RS22330 (4586181) 4586181..4586354 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J8I92_RS22335 (4586382) 4586382..4586678 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J8I92_RS22340 (4586907) 4586907..4587179 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J8I92_RS22345 (4587191) 4587191..4587421 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
J8I92_RS22350 (4587602) 4587602..4589233 + 1632 WP_001216466.1 sensor histidine kinase -
J8I92_RS22355 (4589230) 4589230..4589949 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198307 WP_001173343.1 NZ_CP072688:c4586354-4586181 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198307 NZ_CP096844:3794705-3795115 [Mycobacterium tuberculosis variant bovis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT198307 WP_001312331.1 NZ_CP072688:c4586678-4586382 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT198307 NZ_CP096844:3794430-3794708 [Mycobacterium tuberculosis variant bovis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References