Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4152816..4153366 Replicon chromosome
Accession NZ_CP072688
Organism Escherichia coli strain P4

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag J8I92_RS20235 Protein ID WP_001160966.1
Coordinates 4152816..4153130 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J8I92_RS20240 Protein ID WP_000125566.1
Coordinates 4153133..4153366 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I92_RS20210 (4148491) 4148491..4149777 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J8I92_RS20215 (4149777) 4149777..4150766 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J8I92_RS20220 (4150763) 4150763..4151584 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J8I92_RS20225 (4151587) 4151587..4151964 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J8I92_RS20230 (4151971) 4151971..4152819 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J8I92_RS20235 (4152816) 4152816..4153130 - 315 WP_001160966.1 CcdB family protein Toxin
J8I92_RS20240 (4153133) 4153133..4153366 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J8I92_RS20245 (4153452) 4153452..4153931 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
J8I92_RS20250 (4154123) 4154123..4155985 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
J8I92_RS20255 (4155978) 4155978..4156508 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J8I92_RS20260 (4156616) 4156616..4157947 - 1332 WP_001183207.1 MFS transporter -
J8I92_RS20265 (4158006) 4158006..4158293 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T198306 WP_001160966.1 NZ_CP072688:c4153130-4152816 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T198306 NZ_CP096844:3739331-3739588 [Mycobacterium tuberculosis variant bovis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT198306 WP_000125566.1 NZ_CP072688:c4153366-4153133 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT198306 NZ_CP096844:3739059-3739334 [Mycobacterium tuberculosis variant bovis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References