Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3717018..3717636 Replicon chromosome
Accession NZ_CP072688
Organism Escherichia coli strain P4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J8I92_RS18200 Protein ID WP_001291435.1
Coordinates 3717418..3717636 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J8I92_RS18195 Protein ID WP_000344800.1
Coordinates 3717018..3717392 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I92_RS18185 (3712108) 3712108..3713301 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J8I92_RS18190 (3713324) 3713324..3716473 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J8I92_RS18195 (3717018) 3717018..3717392 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J8I92_RS18200 (3717418) 3717418..3717636 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J8I92_RS18205 (3717809) 3717809..3718360 + 552 WP_000102539.1 maltose O-acetyltransferase -
J8I92_RS18210 (3718476) 3718476..3718946 + 471 WP_000136192.1 YlaC family protein -
J8I92_RS18215 (3719110) 3719110..3720660 + 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J8I92_RS18220 (3720702) 3720702..3721055 - 354 WP_000878142.1 DUF1428 family protein -
J8I92_RS18230 (3721434) 3721434..3721745 + 312 WP_000409908.1 MGMT family protein -
J8I92_RS18235 (3721776) 3721776..3722348 - 573 WP_000779844.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T198305 WP_001291435.1 NZ_CP072688:3717418-3717636 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T198305 NZ_CP096844:c3677789-3677361 [Mycobacterium tuberculosis variant bovis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT198305 WP_000344800.1 NZ_CP072688:3717018-3717392 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT198305 NZ_CP096844:c3678035-3677793 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References