Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 960082..960736 Replicon chromosome
Accession NZ_CP072688
Organism Escherichia coli strain P4

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag J8I92_RS04760 Protein ID WP_000244781.1
Coordinates 960329..960736 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J8I92_RS04755 Protein ID WP_000354046.1
Coordinates 960082..960348 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I92_RS04735 (956170) 956170..957603 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
J8I92_RS04740 (957648) 957648..957959 + 312 WP_001182948.1 N(4)-acetylcytidine aminohydrolase -
J8I92_RS04745 (958123) 958123..958782 + 660 WP_000250274.1 hemolysin III family protein -
J8I92_RS04750 (958859) 958859..959839 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
J8I92_RS04755 (960082) 960082..960348 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J8I92_RS04760 (960329) 960329..960736 + 408 WP_000244781.1 protein YgfX Toxin
J8I92_RS04765 (960776) 960776..961297 - 522 WP_001055874.1 flavodoxin FldB -
J8I92_RS04770 (961409) 961409..962305 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J8I92_RS04775 (962330) 962330..963040 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J8I92_RS04780 (963046) 963046..964779 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T198286 WP_000244781.1 NZ_CP072688:960329-960736 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T198286 NZ_CP096844:c2223946-2223602 [Mycobacterium tuberculosis variant bovis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT198286 WP_000354046.1 NZ_CP072688:960082-960348 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT198286 NZ_CP096844:c2224188-2223940 [Mycobacterium tuberculosis variant bovis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References