Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4106393..4106943 Replicon chromosome
Accession NZ_CP072687
Organism Escherichia coli strain YP3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag J8I95_RS19800 Protein ID WP_001160966.1
Coordinates 4106393..4106707 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J8I95_RS19805 Protein ID WP_000125566.1
Coordinates 4106710..4106943 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I95_RS19775 (4102068) 4102068..4103354 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J8I95_RS19780 (4103354) 4103354..4104343 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J8I95_RS19785 (4104340) 4104340..4105161 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J8I95_RS19790 (4105164) 4105164..4105541 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J8I95_RS19795 (4105548) 4105548..4106396 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J8I95_RS19800 (4106393) 4106393..4106707 - 315 WP_001160966.1 CcdB family protein Toxin
J8I95_RS19805 (4106710) 4106710..4106943 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J8I95_RS19810 (4107029) 4107029..4107508 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
J8I95_RS19815 (4107700) 4107700..4109562 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
J8I95_RS19820 (4109555) 4109555..4110085 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J8I95_RS19825 (4110193) 4110193..4111524 - 1332 WP_001183207.1 MFS transporter -
J8I95_RS19830 (4111583) 4111583..4111870 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T198278 WP_001160966.1 NZ_CP072687:c4106707-4106393 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T198278 NZ_CP096844:1758247-1758651 [Mycobacterium tuberculosis variant bovis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT198278 WP_000125566.1 NZ_CP072687:c4106943-4106710 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT198278 NZ_CP096844:1758038-1758241 [Mycobacterium tuberculosis variant bovis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References