Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4586363..4586860 Replicon chromosome
Accession NZ_CP072686
Organism Escherichia coli strain FP4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J8I94_RS22325 Protein ID WP_001173343.1
Coordinates 4586363..4586536 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag J8I94_RS22330 Protein ID WP_001312331.1
Coordinates 4586564..4586860 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I94_RS22315 (4583033) 4583033..4584490 + 1458 WP_000856828.1 dipeptide/tripeptide permease DtpC -
J8I94_RS22320 (4584727) 4584727..4586244 + 1518 WP_000003805.1 lysine--tRNA ligase -
J8I94_RS22325 (4586363) 4586363..4586536 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J8I94_RS22330 (4586564) 4586564..4586860 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J8I94_RS22335 (4587089) 4587089..4587361 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J8I94_RS22340 (4587373) 4587373..4587603 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
J8I94_RS22345 (4587784) 4587784..4589415 + 1632 WP_001216466.1 sensor histidine kinase -
J8I94_RS22350 (4589412) 4589412..4590131 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198257 WP_001173343.1 NZ_CP072686:c4586536-4586363 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198257 NZ_CP096844:678801-679208 [Mycobacterium tuberculosis variant bovis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT198257 WP_001312331.1 NZ_CP072686:c4586860-4586564 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT198257 NZ_CP096844:678589-678804 [Mycobacterium tuberculosis variant bovis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References