Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3717201..3717819 Replicon chromosome
Accession NZ_CP072686
Organism Escherichia coli strain FP4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J8I94_RS18195 Protein ID WP_001291435.1
Coordinates 3717601..3717819 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J8I94_RS18190 Protein ID WP_000344800.1
Coordinates 3717201..3717575 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I94_RS18180 (3712291) 3712291..3713484 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J8I94_RS18185 (3713507) 3713507..3716656 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J8I94_RS18190 (3717201) 3717201..3717575 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J8I94_RS18195 (3717601) 3717601..3717819 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J8I94_RS18200 (3717992) 3717992..3718543 + 552 WP_000102539.1 maltose O-acetyltransferase -
J8I94_RS18205 (3718659) 3718659..3719129 + 471 WP_000136192.1 YlaC family protein -
J8I94_RS18210 (3719293) 3719293..3720843 + 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J8I94_RS18215 (3720885) 3720885..3721238 - 354 WP_000878142.1 DUF1428 family protein -
J8I94_RS18225 (3721617) 3721617..3721928 + 312 WP_000409908.1 MGMT family protein -
J8I94_RS18230 (3721959) 3721959..3722531 - 573 WP_000779844.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T198255 WP_001291435.1 NZ_CP072686:3717601-3717819 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T198255 NZ_CP096844:364910-365335 [Mycobacterium tuberculosis variant bovis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT198255 WP_000344800.1 NZ_CP072686:3717201-3717575 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT198255 NZ_CP096844:364692-364913 [Mycobacterium tuberculosis variant bovis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References