Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 960081..960735 Replicon chromosome
Accession NZ_CP072686
Organism Escherichia coli strain FP4

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag J8I94_RS04760 Protein ID WP_000244781.1
Coordinates 960328..960735 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J8I94_RS04755 Protein ID WP_000354046.1
Coordinates 960081..960347 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I94_RS04735 (956169) 956169..957602 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
J8I94_RS04740 (957647) 957647..957958 + 312 WP_001182948.1 N(4)-acetylcytidine aminohydrolase -
J8I94_RS04745 (958122) 958122..958781 + 660 WP_000250274.1 hemolysin III family protein -
J8I94_RS04750 (958858) 958858..959838 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
J8I94_RS04755 (960081) 960081..960347 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J8I94_RS04760 (960328) 960328..960735 + 408 WP_000244781.1 protein YgfX Toxin
J8I94_RS04765 (960775) 960775..961296 - 522 WP_001055874.1 flavodoxin FldB -
J8I94_RS04770 (961408) 961408..962304 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J8I94_RS04775 (962329) 962329..963039 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J8I94_RS04780 (963045) 963045..964778 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T198236 WP_000244781.1 NZ_CP072686:960328-960735 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T198236 NZ_CP096843:2831490-2831903 [Mycobacterium tuberculosis variant bovis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT198236 WP_000354046.1 NZ_CP072686:960081-960347 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT198236 NZ_CP096843:2831942-2832199 [Mycobacterium tuberculosis variant bovis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References