Detailed information of TA system TA198235

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 826667..827465
• Replicon: chromosome
• Accession: NZ_CP072686
• Organism: Escherichia coli strain FP4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0HL60
• Locus tag: J8I94_RS04045
• Protein ID: WP_000854692.1
• Coordinates: 826667..827044 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L4NTN5
• Locus tag: J8I94_RS04050
• Protein ID: WP_001605875.1
• Coordinates: 827091..827465 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I94_RS04015 (821806)	821806..822954	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
J8I94_RS04020 (823026)	823026..824009	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
J8I94_RS04025 (824808)	824808..824978	-	171	Protein_791	IS110 family transposase	-
J8I94_RS04030 (825321)	825321..826163	-	843	WP_001280485.1	DUF4942 domain-containing protein	-
J8I94_RS04035 (826248)	826248..826445	-	198	WP_023563519.1	DUF957 domain-containing protein	-
J8I94_RS04040 (826521)	826521..826670	-	150	Protein_794	DUF5983 family protein	-
J8I94_RS04045 (826667)	826667..827044	-	378	WP_000854692.1	TA system toxin CbtA family protein	Toxin
J8I94_RS04050 (827091)	827091..827465	-	375	WP_001605875.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
J8I94_RS04055 (827541)	827541..827762	-	222	WP_000692323.1	DUF987 domain-containing protein	-
J8I94_RS04060 (827831)	827831..828307	-	477	WP_001186181.1	RadC family protein	-
J8I94_RS04065 (828323)	828323..828808	-	486	WP_000213727.1	antirestriction protein	-
J8I94_RS04070 (828900)	828900..829718	-	819	WP_112023519.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	824808..824912	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14119.12 Da
• Isoelectric Point: 7.3249

>T198235 WP_000854692.1 NZ_CP072686:c827044-826667 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWHTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T198235 NZ_CP096843:c2817366-2816947 [Mycobacterium tuberculosis variant bovis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13594.42 Da
• Isoelectric Point: 5.4554

>AT198235 WP_001605875.1 NZ_CP072686:c827465-827091 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT198235 NZ_CP096843:c2817587-2817363 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: A0A1M0HL60

Antitoxin

• AlphaFold DB: A0A1L4NTN5