Detailed information of TA system TA198223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 114441..115351
• Replicon: plasmid unnamed1
• Accession: NZ_CP072654
• Organism: Klebsiella pneumoniae strain KPN234

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: J8J76_RS25760
• Protein ID: WP_004026354.1
• Coordinates: 114441..114911 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: J8J76_RS25765
• Protein ID: WP_004026357.1
• Coordinates: 114908..115351 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8J76_RS25740 (J8J76_25740)	109556..109954	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
J8J76_RS25745 (J8J76_25745)	110291..110785	-	495	WP_004212794.1	thermonuclease family protein	-
J8J76_RS26455	110963..111229	+	267	WP_223175074.1	DUF1173 family protein	-
J8J76_RS25750 (J8J76_25750)	111262..111912	+	651	WP_068893702.1	DUF1173 family protein	-
J8J76_RS25760 (J8J76_25760)	114441..114911	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
J8J76_RS25765 (J8J76_25765)	114908..115351	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
J8J76_RS25770 (J8J76_25770)	115452..115955	-	504	Protein_126	DUF4113 domain-containing protein	-
J8J76_RS25775 (J8J76_25775)	116053..116274	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
J8J76_RS25780 (J8J76_25780)	116455..116574	-	120	Protein_128	Fe3+-citrate ABC transporter substrate-binding protein	-
J8J76_RS25785 (J8J76_25785)	116649..118775	-	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
J8J76_RS25790 (J8J76_25790)	118862..119815	-	954	Protein_130	fec operon regulator FecR	-
J8J76_RS25795 (J8J76_25795)	119812..120333	-	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA	1..210202	210202
	-	flank	IS/Tn	-	-	113298..113801	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T198223 WP_004026354.1 NZ_CP072654:c114911-114441 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T198223 NZ_CP096843:1949567-1949815 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT198223 WP_004026357.1 NZ_CP072654:c115351-114908 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT198223 NZ_CP096843:1949355-1949567 [Mycobacterium tuberculosis variant bovis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9