Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 76947..77683 Replicon plasmid unnamed1
Accession NZ_CP072654
Organism Klebsiella pneumoniae strain KPN234

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag J8J76_RS25570 Protein ID WP_004098919.1
Coordinates 77201..77683 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag J8J76_RS25565 Protein ID WP_004213599.1
Coordinates 76947..77213 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8J76_RS25540 (J8J76_25540) 72179..72736 + 558 WP_004213590.1 recombinase family protein -
J8J76_RS25545 (J8J76_25545) 72739..75708 + 2970 WP_004213592.1 Tn3 family transposase -
J8J76_RS25550 (J8J76_25550) 75750..76244 + 495 WP_004213594.1 hypothetical protein -
J8J76_RS25555 (J8J76_25555) 76305..76508 + 204 WP_004213596.1 HHA domain-containing protein -
J8J76_RS25560 (J8J76_25560) 76522..76752 + 231 WP_004213598.1 hypothetical protein -
J8J76_RS25565 (J8J76_25565) 76947..77213 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
J8J76_RS25570 (J8J76_25570) 77201..77683 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
J8J76_RS25575 (J8J76_25575) 78104..79331 + 1228 Protein_85 IS3 family transposase -
J8J76_RS25580 (J8J76_25580) 79327..79722 - 396 Protein_86 IS3 family transposase -
J8J76_RS25585 (J8J76_25585) 79897..80919 - 1023 WP_004214536.1 porphobilinogen synthase -
J8J76_RS25590 (J8J76_25590) 80983..82329 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA 1..210202 210202
- inside IScluster/Tn - - 72179..79331 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T198222 WP_004098919.1 NZ_CP072654:77201-77683 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T198222 NZ_CP096843:c1929012-1928623 [Mycobacterium tuberculosis variant bovis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT198222 WP_004213599.1 NZ_CP072654:76947-77213 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT198222 NZ_CP096843:c1929236-1929009 [Mycobacterium tuberculosis variant bovis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References