Detailed information of TA system TA198221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19836..20506
• Replicon: plasmid unnamed1
• Accession: NZ_CP072654
• Organism: Klebsiella pneumoniae strain KPN234

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: J8J76_RS25270
• Protein ID: WP_004213072.1
• Coordinates: 20063..20506 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: J8J76_RS25265
• Protein ID: WP_004213073.1
• Coordinates: 19836..20066 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8J76_RS25240 (J8J76_25240)	16059..16958	-	900	WP_004225022.1	nucleotide-binding protein	-
J8J76_RS25245 (J8J76_25245)	16948..17238	-	291	WP_004213078.1	hypothetical protein	-
J8J76_RS25250 (J8J76_25250)	17590..17796	+	207	WP_004213077.1	hypothetical protein	-
J8J76_RS25255 (J8J76_25255)	17786..18079	-	294	WP_004213076.1	hypothetical protein	-
J8J76_RS25260 (J8J76_25260)	18095..19228	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
J8J76_RS25265 (J8J76_25265)	19836..20066	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8J76_RS25270 (J8J76_25270)	20063..20506	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8J76_RS26445	20655..20906	+	252	WP_186987481.1	hypothetical protein	-
J8J76_RS25280 (J8J76_25280)	20929..21233	-	305	Protein_26	transposase	-
J8J76_RS25285 (J8J76_25285)	21650..22286	+	637	Protein_27	mucoid phenotype regulator RmpA2	-
J8J76_RS25290 (J8J76_25290)	22804..23207	-	404	Protein_28	GAF domain-containing protein	-
J8J76_RS25295 (J8J76_25295)	23298..24218	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
J8J76_RS25300 (J8J76_25300)	24267..24758	-	492	WP_011251327.1	DM13 domain-containing protein	-
J8J76_RS25305 (J8J76_25305)	24821..25096	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA	1..210202	210202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T198221 WP_004213072.1 NZ_CP072654:20063-20506 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T198221 NZ_CP096843:1755861-1756265 [Mycobacterium tuberculosis variant bovis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT198221 WP_004213073.1 NZ_CP072654:19836-20066 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT198221 NZ_CP096843:1755652-1755855 [Mycobacterium tuberculosis variant bovis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620