Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5143607..5144232 Replicon chromosome
Accession NZ_CP072653
Organism Klebsiella pneumoniae strain KPN234

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag J8J76_RS24790 Protein ID WP_019705794.1
Coordinates 5143607..5143990 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J8J76_RS24795 Protein ID WP_004150355.1
Coordinates 5143990..5144232 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8J76_RS24775 (J8J76_24775) 5140973..5141875 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J8J76_RS24780 (J8J76_24780) 5141872..5142507 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J8J76_RS24785 (J8J76_24785) 5142504..5143433 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J8J76_RS24790 (J8J76_24790) 5143607..5143990 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
J8J76_RS24795 (J8J76_24795) 5143990..5144232 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J8J76_RS24800 (J8J76_24800) 5144437..5145354 + 918 WP_019705795.1 alpha/beta hydrolase -
J8J76_RS24805 (J8J76_24805) 5145368..5146309 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J8J76_RS24810 (J8J76_24810) 5146354..5146791 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J8J76_RS24815 (J8J76_24815) 5146788..5147648 - 861 WP_069339404.1 virulence factor BrkB family protein -
J8J76_RS24820 (J8J76_24820) 5147642..5148241 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T198220 WP_019705794.1 NZ_CP072653:c5143990-5143607 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T198220 NZ_CP096843:1688028-1688345 [Mycobacterium tuberculosis variant bovis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT198220 WP_004150355.1 NZ_CP072653:c5144232-5143990 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT198220 NZ_CP096843:1687729-1688031 [Mycobacterium tuberculosis variant bovis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References