Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4534623..4535433 Replicon chromosome
Accession NZ_CP072653
Organism Klebsiella pneumoniae strain KPN234

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag J8J76_RS21975 Protein ID WP_004178461.1
Coordinates 4534623..4535156 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J8J76_RS21980 Protein ID WP_002887278.1
Coordinates 4535167..4535433 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8J76_RS21970 (J8J76_21970) 4533454..4534575 + 1122 WP_002887282.1 cupin domain-containing protein -
J8J76_RS21975 (J8J76_21975) 4534623..4535156 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
J8J76_RS21980 (J8J76_21980) 4535167..4535433 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
J8J76_RS21985 (J8J76_21985) 4535536..4536969 - 1434 WP_019705874.1 Cu(+)/Ag(+) sensor histidine kinase -
J8J76_RS21990 (J8J76_21990) 4536959..4537642 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
J8J76_RS21995 (J8J76_21995) 4537814..4539199 + 1386 WP_032409188.1 efflux transporter outer membrane subunit -
J8J76_RS22000 (J8J76_22000) 4539217..4539561 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T198217 WP_004178461.1 NZ_CP072653:c4535156-4534623 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T198217 NZ_CP096843:c1390636-1390343 [Mycobacterium tuberculosis variant bovis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT198217 WP_002887278.1 NZ_CP072653:c4535433-4535167 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT198217 NZ_CP096843:c1390902-1390633 [Mycobacterium tuberculosis variant bovis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References