Detailed information of TA system TA198214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2566301..2567889
• Replicon: chromosome
• Accession: NZ_CP072653
• Organism: Klebsiella pneumoniae strain KPN234

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J8J76_RS12545
• Protein ID: WP_019705665.1
• Coordinates: 2566301..2567623 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J8J76_RS12550
• Protein ID: WP_004176198.1
• Coordinates: 2567623..2567889 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8J76_RS12530 (J8J76_12530)	2561357..2563858	+	2502	WP_064181310.1	fimbria/pilus outer membrane usher protein	-
J8J76_RS12535 (J8J76_12535)	2563846..2564850	+	1005	WP_004184029.1	fimbrial protein	-
J8J76_RS12540 (J8J76_12540)	2564992..2566284	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
J8J76_RS12545 (J8J76_12545)	2566301..2567623	-	1323	WP_019705665.1	type II toxin-antitoxin system HipA family toxin	Toxin
J8J76_RS12550 (J8J76_12550)	2567623..2567889	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J8J76_RS12555 (J8J76_12555)	2568155..2568481	-	327	WP_019705664.1	hydroxyisourate hydrolase	-
J8J76_RS12560 (J8J76_12560)	2568478..2568978	-	501	WP_019705663.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J8J76_RS12565 (J8J76_12565)	2568956..2570335	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
J8J76_RS12570 (J8J76_12570)	2570681..2571835	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
J8J76_RS12575 (J8J76_12575)	2571816..2572739	-	924	WP_019705662.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5358

>T198214 WP_019705665.1 NZ_CP072653:c2567623-2566301 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T198214 NZ_CP096843:c1232692-1232381 [Mycobacterium tuberculosis variant bovis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT198214 WP_004176198.1 NZ_CP072653:c2567889-2567623 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT198214 NZ_CP096843:c1233012-1232692 [Mycobacterium tuberculosis variant bovis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A