Detailed information of TA system TA198200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2180131..2180765
• Replicon: chromosome
• Accession: NZ_CP072652
• Organism: Serratia symbiotica strain 24.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GPZ83_RS11270
• Protein ID: WP_040266910.1
• Coordinates: 2180131..2180307 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GPZ83_RS11275
• Protein ID: WP_160758908.1
• Coordinates: 2180358..2180765 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPZ83_RS11250 (GPZ83_0011245)	2176869..2177585	-	717	Protein_2060	integrase arm-type DNA-binding domain-containing protein	-
GPZ83_RS11260 (GPZ83_0011255)	2178316..2179182	+	867	WP_160744847.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-
GPZ83_RS11265 (GPZ83_0011260)	2179282..2179985	+	704	Protein_2062	IS6 family transposase	-
GPZ83_RS11270 (GPZ83_0011265)	2180131..2180307	+	177	WP_040266910.1	type II toxin-antitoxin system HicA family toxin	Toxin
GPZ83_RS11275 (GPZ83_0011270)	2180358..2180765	+	408	WP_160758908.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GPZ83_RS11280 (GPZ83_0011275)	2180843..2181170	+	328	Protein_2065	phage holin, lambda family	-
GPZ83_RS11285 (GPZ83_0011280)	2181167..2181580	+	414	WP_040266915.1	structural protein	-
GPZ83_RS11290 (GPZ83_0011285)	2181583..2182041	+	459	WP_160744822.1	lysis protein	-
GPZ83_RS11295 (GPZ83_0011290)	2182102..2183061	+	960	WP_040266916.1	hypothetical protein	-
GPZ83_RS11300 (GPZ83_0011295)	2183263..2183541	+	279	WP_160744824.1	hypothetical protein	-
GPZ83_RS11305 (GPZ83_0011300)	2183561..2184603	-	1043	Protein_2070	IS481 family transposase	-
GPZ83_RS15865	2184727..2184954	+	228	Protein_2071	hypothetical protein	-
GPZ83_RS11315 (GPZ83_0011310)	2184959..2185242	+	284	Protein_2072	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2173216..2184309	11093
	-	inside	IScluster/Tn	-	-	2175628..2184561	8933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6619.79 Da
• Isoelectric Point: 11.1475

>T198200 WP_040266910.1 NZ_CP072652:2180131-2180307 [Serratia symbiotica]
VKQSEFRRWLAAQGAEFKDGTNHLKIILNSKQTVMPRHPGKEIPEPLRKAILKQLGIT

Nucleotide

• Length: 177 bp

>T198200 NZ_CP096843:678981-679388 [Mycobacterium tuberculosis variant bovis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14914.08 Da
• Isoelectric Point: 4.6363

>AT198200 WP_160758908.1 NZ_CP072652:2180358-2180765 [Serratia symbiotica]
MRYPVKFEHDETGWCVSFPDIPEALTGGDTREEAIEMAQDALVTAFDFYFEDRRPVPMPSANGEEFIDVPASVAAKVLLL
NAMITTGTTPAQLARRLGTRPQEVNRIVTLNHATKIDTIEAALKALGKRLEITVL

Nucleotide

• Length: 408 bp

>AT198200 NZ_CP096843:678769-678984 [Mycobacterium tuberculosis variant bovis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA