Detailed information of TA system TA198196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 573474..574086
• Replicon: chromosome
• Accession: NZ_CP072652
• Organism: Serratia symbiotica strain 24.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GPZ83_RS03665
• Protein ID: WP_180192808.1
• Coordinates: 573907..574086 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: E9CMQ6
• Locus tag: GPZ83_RS03660
• Protein ID: WP_006709107.1
• Coordinates: 573474..573884 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPZ83_RS03620 (GPZ83_0003615)	568633..568921	+	289	Protein_560	IS110 family transposase	-
GPZ83_RS15585	569165..569332	-	168	WP_226020456.1	hypothetical protein	-
GPZ83_RS15590	569366..569635	-	270	WP_226019949.1	transposase	-
GPZ83_RS15595	569623..570024	-	402	WP_226019950.1	transposase	-
GPZ83_RS03635 (GPZ83_0003630)	570327..571373	-	1047	WP_160758687.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
GPZ83_RS03640 (GPZ83_0003635)	571520..571957	+	438	WP_040263157.1	DNA polymerase III subunit psi	-
GPZ83_RS03645 (GPZ83_0003640)	571902..572345	+	444	WP_040263155.1	ribosomal protein S18-alanine N-acetyltransferase	-
GPZ83_RS03650 (GPZ83_0003645)	572846..573124	+	279	WP_052447629.1	Ig-like domain-containing protein	-
GPZ83_RS03655 (GPZ83_0003650)	573136..573438	+	303	WP_160743757.1	pyocin knob domain-containing protein	-
GPZ83_RS03660 (GPZ83_0003655)	573474..573884	-	411	WP_006709107.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GPZ83_RS03665 (GPZ83_0003660)	573907..574086	-	180	WP_180192808.1	type II toxin-antitoxin system HicA family toxin	Toxin
GPZ83_RS03670 (GPZ83_0003665)	574294..575883	+	1590	WP_040263146.1	peptide chain release factor 3	-
GPZ83_RS03675 (GPZ83_0003670)	576470..577252	+	783	WP_040263144.1	TatD family hydrolase	-
GPZ83_RS03680 (GPZ83_0003675)	577938..578717	+	780	WP_040263142.1	deoxyribose-phosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	562973..574086	11113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6630.83 Da
• Isoelectric Point: 10.9132

>T198196 WP_180192808.1 NZ_CP072652:c574086-573907 [Serratia symbiotica]
MDSRNAIAMVEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T198196 NZ_CP096843:364433-364735 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14742.72 Da
• Isoelectric Point: 4.3299

>AT198196 WP_006709107.1 NZ_CP072652:c573884-573474 [Serratia symbiotica]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESVREAIDAHIELLVEDGEAVPEATSVENWLADPDYAGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRLAADKVIGRAKI

Nucleotide

• Length: 411 bp

>AT198196 NZ_CP096843:364209-364436 [Mycobacterium tuberculosis variant bovis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A068RBW1