Detailed information of TA system TA198183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 479937..480502
• Replicon: chromosome
• Accession: NZ_CP072628
• Organism: Leptospira borgpetersenii strain FMAS_AP3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V6I350
• Locus tag: FH591_RS02060
• Protein ID: WP_002753850.1
• Coordinates: 479937..480185 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FH591_RS02065
• Protein ID: WP_011670965.1
• Coordinates: 480179..480502 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH591_RS02040 (FH591_02040)	474985..475986	+	1002	WP_002732926.1	metallophosphoesterase	-
FH591_RS02045 (FH591_02045)	475989..476666	+	678	WP_002725755.1	hypothetical protein	-
FH591_RS02050 (FH591_02050)	476663..477079	+	417	WP_118883418.1	hypothetical protein	-
FH591_RS02055 (FH591_02055)	477060..479240	+	2181	WP_002732919.1	ATP-dependent DNA helicase RecG	-
FH591_RS02060 (FH591_02060)	479937..480185	+	249	WP_002753850.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
FH591_RS02065 (FH591_02065)	480179..480502	+	324	WP_011670965.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
FH591_RS02070 (FH591_02070)	480938..482473	+	1536	WP_002738394.1	phosphomethylpyrimidine synthase ThiC	-
FH591_RS02075 (FH591_02075)	482849..483505	+	657	Protein_410	helicase-related protein	-
FH591_RS02080 (FH591_02080)	483880..484224	-	345	WP_002728323.1	TIGR04452 family lipoprotein	-
FH591_RS02085 (FH591_02085)	484557..485300	+	744	WP_002728313.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (6-49)

Antitoxin

• mRNA interferase PemK-like (5-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9459.00 Da
• Isoelectric Point: 5.1749

>T198183 WP_002753850.1 NZ_CP072628:479937-480185 [Leptospira borgpetersenii]
MKALVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKSMSSNKDDKLLIPDSIDLSDKDW
EW

Nucleotide

• Length: 249 bp

>T198183 NZ_CP096842:2899839-2900279 [Mycobacterium tuberculosis variant bovis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12296.80 Da
• Isoelectric Point: 10.5161

>AT198183 WP_011670965.1 NZ_CP072628:480179-480502 [Leptospira borgpetersenii]
MVISQYEVYLINLDPTVGHEIKKSRPCVIISPNEMNKTIGTIIITPMTTKSRSYPTRVELTFQGKKGWIVLDQIRTVDKI
RLIKKLGKIDPKTVNKMKLIIKEMLVD

Nucleotide

• Length: 324 bp

>AT198183 NZ_CP096842:2899565-2899852 [Mycobacterium tuberculosis variant bovis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Toxin

• AlphaFold DB: V6I350