Detailed information of TA system TA198180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 479972..480537
• Replicon: chromosome
• Accession: NZ_CP072626
• Organism: Leptospira borgpetersenii strain FMAS_AP4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V6I350
• Locus tag: FH592_RS02060
• Protein ID: WP_002753850.1
• Coordinates: 479972..480220 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FH592_RS02065
• Protein ID: WP_011670965.1
• Coordinates: 480214..480537 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH592_RS02040 (FH592_02040)	475020..476021	+	1002	WP_002732926.1	metallophosphoesterase	-
FH592_RS02045 (FH592_02045)	476024..476701	+	678	WP_002725755.1	hypothetical protein	-
FH592_RS02050 (FH592_02050)	476698..477114	+	417	WP_118883418.1	hypothetical protein	-
FH592_RS02055 (FH592_02055)	477095..479275	+	2181	WP_002732919.1	ATP-dependent DNA helicase RecG	-
FH592_RS02060 (FH592_02060)	479972..480220	+	249	WP_002753850.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
FH592_RS02065 (FH592_02065)	480214..480537	+	324	WP_011670965.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
FH592_RS02070 (FH592_02070)	480973..482508	+	1536	WP_002738394.1	phosphomethylpyrimidine synthase ThiC	-
FH592_RS02075 (FH592_02075)	482884..483540	+	657	Protein_411	helicase-related protein	-
FH592_RS02080 (FH592_02080)	483915..484259	-	345	WP_002728323.1	TIGR04452 family lipoprotein	-
FH592_RS02085 (FH592_02085)	484592..485335	+	744	WP_002728313.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (6-49)

Antitoxin

• mRNA interferase PemK-like (5-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9459.00 Da
• Isoelectric Point: 5.1749

>T198180 WP_002753850.1 NZ_CP072626:479972-480220 [Leptospira borgpetersenii]
MKALVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKSMSSNKDDKLLIPDSIDLSDKDW
EW

Nucleotide

• Length: 249 bp

>T198180 NZ_CP072626:479972-480220 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12296.80 Da
• Isoelectric Point: 10.5161

>AT198180 WP_011670965.1 NZ_CP072626:480214-480537 [Leptospira borgpetersenii]
MVISQYEVYLINLDPTVGHEIKKSRPCVIISPNEMNKTIGTIIITPMTTKSRSYPTRVELTFQGKKGWIVLDQIRTVDKI
RLIKKLGKIDPKTVNKMKLIIKEMLVD

Nucleotide

• Length: 324 bp

>AT198180 NZ_CP072626:480214-480537 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTAGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Structures

Toxin

• AlphaFold DB: V6I350