Detailed information of TA system TA198177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 480384..480949
• Replicon: chromosome
• Accession: NZ_CP072624
• Organism: Leptospira borgpetersenii strain FMAS_PN1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V6I350
• Locus tag: FH596_RS02065
• Protein ID: WP_002753850.1
• Coordinates: 480384..480632 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FH596_RS02070
• Protein ID: WP_011670965.1
• Coordinates: 480626..480949 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH596_RS02045 (FH596_02045)	475432..476433	+	1002	WP_002732926.1	metallophosphoesterase	-
FH596_RS02050 (FH596_02050)	476436..477113	+	678	WP_002725755.1	hypothetical protein	-
FH596_RS02055 (FH596_02055)	477110..477526	+	417	WP_118883418.1	hypothetical protein	-
FH596_RS02060 (FH596_02060)	477507..479687	+	2181	WP_002732919.1	ATP-dependent DNA helicase RecG	-
FH596_RS02065 (FH596_02065)	480384..480632	+	249	WP_002753850.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
FH596_RS02070 (FH596_02070)	480626..480949	+	324	WP_011670965.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
FH596_RS02075 (FH596_02075)	481385..482920	+	1536	WP_002738394.1	phosphomethylpyrimidine synthase ThiC	-
FH596_RS02080 (FH596_02080)	483296..483952	+	657	Protein_411	helicase-related protein	-
FH596_RS02085 (FH596_02085)	484327..484671	-	345	WP_002728323.1	TIGR04452 family lipoprotein	-
FH596_RS02090 (FH596_02090)	485004..485747	+	744	WP_002728313.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (6-49)

Antitoxin

• mRNA interferase PemK-like (5-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9459.00 Da
• Isoelectric Point: 5.1749

>T198177 WP_002753850.1 NZ_CP072624:480384-480632 [Leptospira borgpetersenii]
MKALVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKSMSSNKDDKLLIPDSIDLSDKDW
EW

Nucleotide

• Length: 249 bp

>T198177 NZ_CP096842:c2387594-2387277 [Mycobacterium tuberculosis variant bovis]
ATGACGCGTAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12296.80 Da
• Isoelectric Point: 10.5161

>AT198177 WP_011670965.1 NZ_CP072624:480626-480949 [Leptospira borgpetersenii]
MVISQYEVYLINLDPTVGHEIKKSRPCVIISPNEMNKTIGTIIITPMTTKSRSYPTRVELTFQGKKGWIVLDQIRTVDKI
RLIKKLGKIDPKTVNKMKLIIKEMLVD

Nucleotide

• Length: 324 bp

>AT198177 NZ_CP096842:c2387806-2387591 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: V6I350