Detailed information of TA system TA198169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 2829473..2830038
• Replicon: chromosome
• Accession: NZ_CP072618
• Organism: Leptospira borgpetersenii strain FMAS_AP9

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FH601_RS12185
• Protein ID: WP_011670965.1
• Coordinates: 2829473..2829796 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: V6I350
• Locus tag: FH601_RS12190
• Protein ID: WP_002753850.1
• Coordinates: 2829790..2830038 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH601_RS12165 (FH601_12165)	2824675..2825418	-	744	WP_002728313.1	M15 family metallopeptidase	-
FH601_RS12170 (FH601_12170)	2825751..2826095	+	345	WP_002728323.1	TIGR04452 family lipoprotein	-
FH601_RS12175 (FH601_12175)	2826470..2827126	-	657	Protein_2401	helicase-related protein	-
FH601_RS12180 (FH601_12180)	2827502..2829037	-	1536	WP_002738394.1	phosphomethylpyrimidine synthase ThiC	-
FH601_RS12185 (FH601_12185)	2829473..2829796	-	324	WP_011670965.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FH601_RS12190 (FH601_12190)	2829790..2830038	-	249	WP_002753850.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH601_RS12195 (FH601_12195)	2830735..2832915	-	2181	WP_002732919.1	ATP-dependent DNA helicase RecG	-
FH601_RS12200 (FH601_12200)	2832896..2833312	-	417	WP_118883418.1	hypothetical protein	-
FH601_RS12205 (FH601_12205)	2833309..2833986	-	678	WP_002725755.1	hypothetical protein	-
FH601_RS12210 (FH601_12210)	2833989..2834990	-	1002	WP_002732926.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-104)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12296.80 Da
• Isoelectric Point: 10.5161

>T198169 WP_011670965.1 NZ_CP072618:c2829796-2829473 [Leptospira borgpetersenii]
MVISQYEVYLINLDPTVGHEIKKSRPCVIISPNEMNKTIGTIIITPMTTKSRSYPTRVELTFQGKKGWIVLDQIRTVDKI
RLIKKLGKIDPKTVNKMKLIIKEMLVD

Nucleotide

• Length: 324 bp

>T198169 NZ_CP096842:2197918-2198295 [Mycobacterium tuberculosis variant bovis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9459.00 Da
• Isoelectric Point: 5.1749

>AT198169 WP_002753850.1 NZ_CP072618:c2830038-2829790 [Leptospira borgpetersenii]
MKALVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKSMSSNKDDKLLIPDSIDLSDKDW
EW

Nucleotide

• Length: 249 bp

>AT198169 NZ_CP096842:2198337-2198786 [Mycobacterium tuberculosis variant bovis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Antitoxin

• AlphaFold DB: V6I350