Detailed information of TA system TA198166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3432195..3433112
• Replicon: chromosome
• Accession: NZ_CP072612
• Organism: Bacillus amyloliquefaciens strain G10

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: J8G07_RS16725
• Protein ID: WP_007407256.1
• Coordinates: 3432195..3432941 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: J8G07_RS16730
• Protein ID: WP_003154807.1
• Coordinates: 3432942..3433112 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8G07_RS16705	3427588..3428538	-	951	WP_032874618.1	ring-cleaving dioxygenase	-
J8G07_RS16710	3428860..3430176	+	1317	WP_032874616.1	amino acid permease	-
J8G07_RS16715	3430462..3431079	+	618	WP_032874614.1	DUF47 domain-containing protein	-
J8G07_RS16720	3431092..3432090	+	999	WP_032874611.1	inorganic phosphate transporter	-
J8G07_RS16725	3432195..3432941	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J8G07_RS16730	3432942..3433112	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
J8G07_RS19575	3433209..3433334	+	126	WP_003154809.1	hypothetical protein	-
J8G07_RS16735	3433369..3434247	-	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
J8G07_RS16740	3434261..3434524	-	264	WP_003154813.1	phage holin	-
J8G07_RS16745	3434538..3434801	-	264	WP_003154815.1	hemolysin XhlA family protein	-
J8G07_RS16750	3434853..3435614	-	762	WP_032874607.1	hypothetical protein	-
J8G07_RS16755	3435671..3435868	-	198	WP_032874605.1	XkdX family protein	-
J8G07_RS16760	3435873..3436244	-	372	WP_032874603.1	XkdW family protein	-
J8G07_RS16765	3436257..3437879	-	1623	WP_032874601.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3423920..3476866	52946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T198166 WP_007407256.1 NZ_CP072612:3432195-3432941 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T198166 NZ_CP096842:c1937686-1937297 [Mycobacterium tuberculosis variant bovis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT198166 WP_003154807.1 NZ_CP072612:3432942-3433112 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT198166 NZ_CP096842:c1937910-1937683 [Mycobacterium tuberculosis variant bovis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14