Detailed information of TA system TA198165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 254060..254697
• Replicon: chromosome
• Accession: NZ_CP072612
• Organism: Bacillus amyloliquefaciens strain G10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: J8G07_RS01295
• Protein ID: WP_003156187.1
• Coordinates: 254060..254410 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: J8G07_RS01300
• Protein ID: WP_003156188.1
• Coordinates: 254416..254697 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8G07_RS01255	249100..249702	-	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-
J8G07_RS01260	249702..250490	-	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
J8G07_RS01265	250456..250938	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
J8G07_RS01270	250935..251264	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
J8G07_RS01275	251328..252335	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
J8G07_RS01280	252347..252748	-	402	WP_003156178.1	anti-sigma regulatory factor	-
J8G07_RS01285	252751..253116	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
J8G07_RS01290	253121..253942	-	822	WP_003156182.1	STAS domain-containing protein	-
J8G07_RS01295	254060..254410	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
J8G07_RS01300	254416..254697	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
J8G07_RS01305	254817..255986	-	1170	WP_032873001.1	alanine racemase	-
J8G07_RS01310	256103..257110	-	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
J8G07_RS01315	257275..257640	-	366	WP_007609580.1	holo-ACP synthase	-
J8G07_RS01320	257733..258332	+	600	WP_032872997.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T198165 WP_003156187.1 NZ_CP072612:c254410-254060 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T198165 NZ_CP096842:1755277-1755681 [Mycobacterium tuberculosis variant bovis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT198165 WP_003156188.1 NZ_CP072612:c254697-254416 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT198165 NZ_CP096842:1755068-1755271 [Mycobacterium tuberculosis variant bovis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5