Detailed information of TA system TA198161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2167517..2168151
• Replicon: chromosome
• Accession: NZ_CP072610
• Organism: Pseudomonas wenzhouensis strain A20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L1LRL5
• Locus tag: J7655_RS09960
• Protein ID: WP_009408500.1
• Coordinates: 2167747..2168151 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J7655_RS09955
• Protein ID: WP_057391045.1
• Coordinates: 2167517..2167747 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7655_RS09930 (J7655_09905)	2163565..2164551	+	987	WP_230927599.1	hypothetical protein	-
J7655_RS09935	2164654..2164836	-	183	WP_230927600.1	hypothetical protein	-
J7655_RS09940 (J7655_09910)	2164846..2165343	-	498	WP_230927601.1	DNA repair protein RadC	-
J7655_RS09945 (J7655_09915)	2165315..2166265	-	951	WP_230927602.1	hydrolase or metal-binding protein	-
J7655_RS09950 (J7655_09920)	2166356..2167360	-	1005	WP_230927603.1	YqaJ viral recombinase family protein	-
J7655_RS09955 (J7655_09925)	2167517..2167747	+	231	WP_057391045.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J7655_RS09960 (J7655_09930)	2167747..2168151	+	405	WP_009408500.1	tRNA(fMet)-specific endonuclease VapC	Toxin
J7655_RS09965 (J7655_09935)	2168238..2169206	-	969	WP_230927604.1	DUF932 domain-containing protein	-
J7655_RS09970	2169266..2169598	-	333	WP_036992178.1	hypothetical protein	-
J7655_RS09975 (J7655_09940)	2170001..2170273	+	273	WP_096862010.1	CopG family ribbon-helix-helix protein	-
J7655_RS09980 (J7655_09945)	2170261..2170554	+	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J7655_RS09985 (J7655_09950)	2170548..2171402	-	855	Protein_1959	site-specific integrase	-
J7655_RS09990 (J7655_09955)	2171403..2171567	-	165	Protein_1960	helix-turn-helix domain-containing protein	-
J7655_RS09995 (J7655_09960)	2171713..2172144	+	432	WP_230924495.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2120834..2202358	81524
	-	flank	IS/Tn	-	-	2171713..2172144	431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14977.20 Da
• Isoelectric Point: 7.2774

>T198161 WP_009408500.1 NZ_CP072610:2167747-2168151 [Pseudomonas wenzhouensis]
MLKYMLDTNICIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVNA

Nucleotide

• Length: 405 bp

>T198161 NZ_CP096842:c1390011-1389718 [Mycobacterium tuberculosis variant bovis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8446.44 Da
• Isoelectric Point: 4.2683

>AT198161 WP_057391045.1 NZ_CP072610:2167517-2167747 [Pseudomonas wenzhouensis]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDLWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT198161 NZ_CP096842:c1390277-1390008 [Mycobacterium tuberculosis variant bovis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: L1LRL5