Detailed information of TA system TA198160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1160463..1162054
• Replicon: chromosome
• Accession: NZ_CP072610
• Organism: Pseudomonas wenzhouensis strain A20

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J7655_RS05300
• Protein ID: WP_230926879.1
• Coordinates: 1160463..1161788 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J7655_RS05305
• Protein ID: WP_230926880.1
• Coordinates: 1161788..1162054 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7655_RS05280 (J7655_05265)	1155603..1156457	-	855	Protein_1040	IS5 family transposase	-
J7655_RS05285 (J7655_05270)	1156666..1157649	-	984	WP_230926877.1	helix-turn-helix domain-containing protein	-
J7655_RS05290 (J7655_05275)	1157746..1158177	-	432	WP_230926878.1	hypothetical protein	-
J7655_RS05295 (J7655_05280)	1159190..1159978	-	789	Protein_1043	LysR family transcriptional regulator	-
J7655_RS05300 (J7655_05285)	1160463..1161788	+	1326	WP_230926879.1	HipA domain-containing protein	Toxin
J7655_RS05305 (J7655_05290)	1161788..1162054	+	267	WP_230926880.1	helix-turn-helix transcriptional regulator	Antitoxin
J7655_RS05310 (J7655_05295)	1162106..1162909	+	804	WP_230926881.1	inositol monophosphatase family protein	-
J7655_RS05315 (J7655_05300)	1163212..1163379	+	168	Protein_1047	IS5/IS1182 family transposase	-
J7655_RS05320 (J7655_05305)	1163462..1165033	+	1572	WP_230926882.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1151505..1163382	11877
	-	flank	IS/Tn	-	-	1163212..1163382	170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-77)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 49124.66 Da
• Isoelectric Point: 5.2211

>T198160 WP_230926879.1 NZ_CP072610:1160463-1161788 [Pseudomonas wenzhouensis]
MHNLTLQVYRDGQWRNAMELRFEQPEEGLEGACAYGYQQGYLVDALESVGSRLNVAVSAAYPLGWDSWRDKKAPAFLFDI
LPSGAARRFLLKRLGGERPEGLNLDLYLLGRCTPAPIGNLRIKESVEAIAGSPVLGFTRDEVISRDSRFLEYAYEQGAAI
GGATGAGGEAPKLLLTEDRQGALHPDAVLPDADAAQHWFVKFARNKASQTDQDILRSEYCFYRAVQQLGMDTVAAEGLAL
EEGNKPSLWMHRFDREISPAGVSRTAVESMYSLCGVTEAGSYMLHTHVLGNLVELWRAVGQEQQVGDLVFEYMRRDLLNQ
ILGNSDNHGRNSAILRCEQGVRLAPIYDLAPMVMDEEGVTRTTKWPKHIELAGEVNWRRACDEVAGWVNADELYQRLRLA
AQDLLALPDLLSAQGLPDSTMSHPRIALRNLRQRLNQWGLV

Nucleotide

• Length: 1326 bp

>T198160 NZ_CP096842:1385568-1385999 [Mycobacterium tuberculosis variant bovis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10002.66 Da
• Isoelectric Point: 11.3500

>AT198160 WP_230926880.1 NZ_CP072610:1161788-1162054 [Pseudomonas wenzhouensis]
MDILERNRILNELQQQLASGEITIGGAVRRLRKEVTGLQQARFAQMCKLSLRALRQLELDESNPTVQTLNRVFNPFGMQV
GIVPKTPR

Nucleotide

• Length: 267 bp

>AT198160 NZ_CP096842:1385311-1385571 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA