Detailed information of TA system TA198145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 5617138..5617654
• Replicon: chromosome
• Accession: NZ_CP072600
• Organism: Leptothermofonsia sichuanensis PKUAC-SCTE412

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: J5X98_RS24245
• Protein ID: WP_223047596.1
• Coordinates: 5617379..5617654 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: J5X98_RS24240
• Protein ID: WP_233745979.1
• Coordinates: 5617138..5617359 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5X98_RS24225 (J5X98_24210)	5612254..5613573	+	1320	WP_223047592.1	dihydroorotase	-
J5X98_RS24230 (J5X98_24215)	5613824..5615872	+	2049	WP_223047593.1	elongation factor G	-
J5X98_RS24235 (J5X98_24220)	5616522..5617133	-	612	WP_223047594.1	hypothetical protein	-
J5X98_RS24240 (J5X98_24225)	5617138..5617359	-	222	WP_233745979.1	BrnA antitoxin family protein	Antitoxin
J5X98_RS24245 (J5X98_24230)	5617379..5617654	-	276	WP_223047596.1	BrnT family toxin	Toxin
J5X98_RS24250 (J5X98_24235)	5617930..5618892	+	963	WP_223047597.1	protochlorophyllide reductase	-
J5X98_RS24255 (J5X98_24240)	5619407..5619688	-	282	WP_223047598.1	hypothetical protein	-
J5X98_RS24260 (J5X98_24245)	5620045..5620716	+	672	WP_223047599.1	hypothetical protein	-
J5X98_RS24265 (J5X98_24250)	5620853..5621485	+	633	WP_223047600.1	Crp/Fnr family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (10-68)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10680.91 Da
• Isoelectric Point: 4.8668

>T198145 WP_223047596.1 NZ_CP072600:c5617654-5617379 [Leptothermofonsia sichuanensis PKUAC-SCTE412]
MEYEWDEAKRLANLRKHGIDFIDVPAIFGGDTVTVEDDRYSYGEQRFITFGLLQGRVIAVVHTEQEDYTRIISARKATKY
EQRAYFEQLSN

Nucleotide

• Length: 276 bp

>T198145 NZ_CP096842:c696212-695820 [Mycobacterium tuberculosis variant bovis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8430.60 Da
• Isoelectric Point: 5.7783

>AT198145 WP_233745979.1 NZ_CP072600:c5617359-5617138 [Leptothermofonsia sichuanensis PKUAC-SCTE412]
MTDEDIDFSDCPEVTPEMFAKAIVRRGLPVAKAKTQVTLRIDSDVLDWFKSQGRGYQTQINQLLRAYMEAHQR

Nucleotide

• Length: 222 bp

>AT198145 NZ_CP096842:c696466-696209 [Mycobacterium tuberculosis variant bovis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA