Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 19325..20462 Replicon plasmid pVB976_p3
Accession NZ_CP072589
Organism Enterococcus faecium strain VB976

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag J7300_RS15705 Protein ID WP_001284311.1
Coordinates 19599..20462 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J7300_RS15700 Protein ID WP_000301765.1
Coordinates 19325..19597 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7300_RS15670 (J7300_15710) 15135..15707 - 573 WP_002303206.1 recombinase family protein -
J7300_RS15675 (J7300_15715) 15723..16328 - 606 WP_000599739.1 Fic family protein -
J7300_RS15680 (J7300_15720) 16526..17206 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
J7300_RS15685 (J7300_15725) 17682..18272 + 591 Protein_20 MobV family relaxase -
J7300_RS15690 (J7300_15730) 18347..19027 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
J7300_RS15695 (J7300_15735) 19093..19308 + 216 WP_001835296.1 peptide-binding protein -
J7300_RS15700 (J7300_15740) 19325..19597 + 273 WP_000301765.1 antitoxin Antitoxin
J7300_RS15705 (J7300_15745) 19599..20462 + 864 WP_001284311.1 zeta toxin family protein Toxin
J7300_RS15710 (J7300_15750) 20725..21462 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
J7300_RS15715 (J7300_15755) 21587..21670 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
J7300_RS16170 (J7300_15760) 21719..21802 - 84 Protein_27 MLS leader peptide -
J7300_RS15725 (J7300_15765) 22212..23006 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
J7300_RS15730 (J7300_15770) 23099..23350 - 252 Protein_29 GNAT family N-acetyltransferase -
J7300_RS15735 (J7300_15775) 23595..24890 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia - 1..29437 29437
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia - 1446..27055 25609


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T198136 WP_001284311.1 NZ_CP072589:19599-20462 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T198136 NZ_CP096841:3721833-3722090 [Mycobacterium tuberculosis variant bovis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT198136 WP_000301765.1 NZ_CP072589:19325-19597 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT198136 NZ_CP096841:3721561-3721836 [Mycobacterium tuberculosis variant bovis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References