Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 165333..165852 Replicon plasmid pVB976_p1
Accession NZ_CP072587
Organism Enterococcus faecium strain VB976

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag J7300_RS14375 Protein ID WP_000588503.1
Coordinates 165333..165590 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag J7300_RS14380 Protein ID WP_000388479.1
Coordinates 165583..165852 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7300_RS14340 (J7300_14365) 160359..160943 - 585 WP_002299575.1 recombinase family protein -
J7300_RS14345 (J7300_14370) 161154..161357 - 204 WP_002299573.1 hypothetical protein -
J7300_RS14350 (J7300_14375) 161367..161789 - 423 WP_002303116.1 hypothetical protein -
J7300_RS14355 (J7300_14380) 162027..162641 + 615 WP_001261742.1 recombinase family protein -
J7300_RS14360 (J7300_14385) 162806..163630 - 825 WP_001809248.1 hypothetical protein -
J7300_RS14365 (J7300_14390) 163654..163809 - 156 WP_002347142.1 hypothetical protein -
J7300_RS14370 (J7300_14395) 163871..164830 - 960 WP_002287225.1 IS30 family transposase -
J7300_RS14375 (J7300_14400) 165333..165590 - 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
J7300_RS14380 (J7300_14405) 165583..165852 - 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J7300_RS14385 (J7300_14410) 165983..166162 - 180 WP_001120991.1 hypothetical protein -
J7300_RS16090 166178..166378 - 201 WP_002347143.1 hypothetical protein -
J7300_RS14390 (J7300_14415) 166543..167250 - 708 WP_002303117.1 hypothetical protein -
J7300_RS14395 (J7300_14420) 167243..167680 - 438 WP_002300851.1 hypothetical protein -
J7300_RS14400 (J7300_14425) 167695..167946 - 252 WP_002296238.1 hypothetical protein -
J7300_RS14405 (J7300_14430) 167946..168152 - 207 WP_002347218.1 hypothetical protein -
J7300_RS14410 (J7300_14435) 168589..168849 - 261 WP_002295623.1 hypothetical protein -
J7300_RS16095 169023..169127 + 105 WP_008274030.1 putative holin-like toxin -
J7300_RS14420 (J7300_14445) 169268..169753 - 486 WP_002303313.1 single-stranded DNA-binding protein -
J7300_RS14425 (J7300_14450) 169756..170655 - 900 WP_002303312.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..242945 242945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T198134 WP_000588503.1 NZ_CP072587:c165590-165333 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T198134 NZ_CP096841:3504876-3505220 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT198134 WP_000388479.1 NZ_CP072587:c165852-165583 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT198134 NZ_CP096841:3505217-3505546 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References