Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicB-HicA |
| Location | 16989..17606 | Replicon | plasmid pVB976_p1 |
| Accession | NZ_CP072587 | ||
| Organism | Enterococcus faecium strain VB976 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | - |
| Locus tag | J7300_RS13595 | Protein ID | WP_224451579.1 |
| Coordinates | 16989..17141 (+) | Length | 51 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A829EWE8 |
| Locus tag | J7300_RS13600 | Protein ID | WP_002305052.1 |
| Coordinates | 17175..17606 (+) | Length | 144 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J7300_RS13550 (J7300_13560) | 12830..13315 | + | 486 | Protein_10 | single-stranded DNA-binding protein | - |
| J7300_RS13555 (J7300_13565) | 13337..13594 | + | 258 | WP_002295625.1 | hypothetical protein | - |
| J7300_RS13560 (J7300_13570) | 13753..13897 | - | 145 | Protein_12 | type I toxin-antitoxin system toxin PepG1 | - |
| J7300_RS13565 (J7300_13575) | 14089..14289 | + | 201 | WP_227005125.1 | hypothetical protein | - |
| J7300_RS13570 (J7300_13580) | 14737..14943 | + | 207 | WP_002324499.1 | hypothetical protein | - |
| J7300_RS13575 (J7300_13585) | 14943..15194 | + | 252 | WP_002287517.1 | hypothetical protein | - |
| J7300_RS13580 (J7300_13590) | 15210..15647 | + | 438 | WP_002305049.1 | hypothetical protein | - |
| J7300_RS13585 (J7300_13595) | 15640..16335 | + | 696 | WP_002305050.1 | hypothetical protein | - |
| J7300_RS16055 | 16500..16700 | + | 201 | WP_002324500.1 | hypothetical protein | - |
| J7300_RS13590 (J7300_13600) | 16716..16895 | + | 180 | Protein_19 | hypothetical protein | - |
| J7300_RS13595 (J7300_13605) | 16989..17141 | + | 153 | WP_224451579.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| J7300_RS13600 (J7300_13610) | 17175..17606 | + | 432 | WP_002305052.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| J7300_RS13605 (J7300_13615) | 17780..18733 | - | 954 | WP_101699768.1 | IS30 family transposase | - |
| J7300_RS13610 (J7300_13620) | 19029..19901 | - | 873 | WP_002292418.1 | ROK family protein | - |
| J7300_RS13615 (J7300_13625) | 20165..22111 | - | 1947 | WP_002303302.1 | sucrose-specific PTS transporter subunit IIBC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-aph(2'') | - | 1..242945 | 242945 | |
| - | flank | IS/Tn | - | - | 17780..18733 | 953 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 51 a.a. Molecular weight: 5815.65 Da Isoelectric Point: 10.3560
>T198133 WP_224451579.1 NZ_CP072587:16989-17141 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK
Download Length: 153 bp
>T198133 NZ_CP096841:3136655-3137098 [Mycobacterium tuberculosis variant bovis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA
Antitoxin
Download Length: 144 a.a. Molecular weight: 15811.92 Da Isoelectric Point: 4.1638
>AT198133 WP_002305052.1 NZ_CP072587:17175-17606 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN
Download Length: 432 bp
>AT198133 NZ_CP096841:3136411-3136668 [Mycobacterium tuberculosis variant bovis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|