Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 479630..480201 Replicon chromosome
Accession NZ_CP072586
Organism Enterococcus faecium strain VB976

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag J7300_RS02280 Protein ID WP_002286801.1
Coordinates 479860..480201 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J7300_RS02275 Protein ID WP_002323011.1
Coordinates 479630..479860 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7300_RS02245 (J7300_02250) 474763..476094 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
J7300_RS02250 (J7300_02255) 476116..476742 + 627 WP_002286816.1 cysteine hydrolase -
J7300_RS02255 (J7300_02260) 476925..477506 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
J7300_RS15925 478238..478800 + 563 Protein_448 SOS response-associated peptidase family protein -
J7300_RS02270 (J7300_02275) 479005..479343 - 339 WP_002286804.1 hypothetical protein -
J7300_RS02275 (J7300_02280) 479630..479860 + 231 WP_002323011.1 hypothetical protein Antitoxin
J7300_RS02280 (J7300_02285) 479860..480201 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J7300_RS02285 (J7300_02290) 481051..481236 + 186 WP_002304207.1 hypothetical protein -
J7300_RS02290 (J7300_02295) 481741..481935 + 195 WP_002297028.1 hypothetical protein -
J7300_RS02295 (J7300_02300) 482126..482375 + 250 Protein_454 transposase -
J7300_RS02300 (J7300_02305) 482619..483449 - 831 WP_002286789.1 manganese catalase family protein -
J7300_RS02305 (J7300_02310) 483657..484991 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T198132 WP_002286801.1 NZ_CP072586:479860-480201 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T198132 NZ_CP096841:3131095-3131358 [Mycobacterium tuberculosis variant bovis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT198132 WP_002323011.1 NZ_CP072586:479630-479860 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT198132 NZ_CP096841:3130810-3131091 [Mycobacterium tuberculosis variant bovis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References