198130

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 7276..8384 Replicon plasmid pVB12993_p3
Accession NZ_CP072582
Organism Enterococcus faecium strain VB12993

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag J7299_RS15330 Protein ID WP_002303392.1
Coordinates 7276..8145 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag J7299_RS15335 Protein ID WP_002303393.1
Coordinates 8160..8384 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7299_RS15300 (J7299_15300) 2572..2823 - 252 Protein_4 GNAT family N-acetyltransferase -
J7299_RS15305 (J7299_15305) 3068..4363 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
J7299_RS15310 (J7299_15310) 4464..5372 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
J7299_RS15315 (J7299_15315) 5396..5623 - 228 Protein_7 hypothetical protein -
J7299_RS15320 (J7299_15320) 5620..6528 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
J7299_RS15325 (J7299_15325) 6561..7295 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
J7299_RS15330 (J7299_15330) 7276..8145 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
J7299_RS15335 (J7299_15335) 8160..8384 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
J7299_RS15340 (J7299_15340) 8527..9348 - 822 Protein_12 recombinase zinc beta ribbon domain-containing protein -
J7299_RS15345 (J7299_15345) 10036..10593 - 558 Protein_13 lincosamide nucleotidyltransferase Lnu(B) -
J7299_RS15350 (J7299_15350) 10668..11348 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
J7299_RS15355 (J7299_15355) 11358..11828 + 471 Protein_15 protein rep -
J7299_RS15360 (J7299_15360) 11862..12542 - 681 WP_194849700.1 IS6-like element IS1216 family transposase -
J7299_RS15365 (J7299_15365) 12975..13187 - 213 WP_002324171.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia - 1..32740 32740
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia - 198..31240 31042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T198130 WP_002303392.1 NZ_CP072582:c8145-7276 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T198130 NZ_CP096841:c3087831-3086947 [Mycobacterium tuberculosis variant bovis]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACTGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT198130 WP_002303393.1 NZ_CP072582:c8384-8160 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT198130 NZ_CP096841:c3088721-3087834 [Mycobacterium tuberculosis variant bovis]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGCTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGTGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGACCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGTCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References