Detailed information of TA system TA198129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 25512..26031
• Replicon: plasmid pVB12993_p2
• Accession: NZ_CP072581
• Organism: Enterococcus faecium strain VB12993

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: J7299_RS15220
• Protein ID: WP_000588503.1
• Coordinates: 25774..26031 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: J7299_RS15215
• Protein ID: WP_000388479.1
• Coordinates: 25512..25781 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7299_RS15180 (J7299_15180)	21083..21499	-	417	WP_001232859.1	hypothetical protein	-
J7299_RS15185 (J7299_15185)	21502..21786	-	285	WP_000353844.1	hypothetical protein	-
J7299_RS15190 (J7299_15190)	22152..22376	+	225	WP_000410576.1	hypothetical protein	-
J7299_RS15200 (J7299_15200)	23499..24179	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
J7299_RS15205 (J7299_15205)	24255..24821	+	567	Protein_29	hypothetical protein	-
J7299_RS15760	24986..25186	+	201	WP_001033544.1	4-hydroxybenzoate octaprenyltransferase	-
J7299_RS15210 (J7299_15210)	25202..25381	+	180	WP_001120991.1	hypothetical protein	-
J7299_RS15215 (J7299_15215)	25512..25781	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J7299_RS15220 (J7299_15220)	25774..26031	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
J7299_RS15225 (J7299_15225)	26669..27277	+	609	WP_224752223.1	hypothetical protein	-
J7299_RS15230 (J7299_15230)	27411..28730	+	1320	WP_000202380.1	IS1380-like element IS1678 family transposase	-
J7299_RS15235 (J7299_15235)	28943..29176	+	234	WP_002296232.1	hypothetical protein	-
J7299_RS15240 (J7299_15240)	29357..30544	-	1188	WP_002296840.1	IS256-like element IS16 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III / VanHAX	-	1..35966	35966
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia / aph(3')-III / VanHAX	-	1657..30544	28887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T198129 WP_000588503.1 NZ_CP072581:25774-26031 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T198129 NZ_CP096841:c3065742-3065386 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT198129 WP_000388479.1 NZ_CP072581:25512-25781 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT198129 NZ_CP096841:c3065956-3065726 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7