Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 18865..19472 Replicon plasmid pVB12993_p1
Accession NZ_CP072580
Organism Enterococcus faecium strain VB12993

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag J7299_RS13885 Protein ID WP_002321032.1
Coordinates 19122..19472 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag J7299_RS13880 Protein ID WP_002287514.1
Coordinates 18865..19128 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7299_RS13840 (J7299_13840) 13901..14800 + 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
J7299_RS13845 (J7299_13845) 14803..15288 + 486 WP_002296241.1 single-stranded DNA-binding protein -
J7299_RS13850 (J7299_13850) 15654..15914 + 261 WP_002296240.1 hypothetical protein -
J7299_RS13855 (J7299_13855) 16355..16561 + 207 WP_002296239.1 hypothetical protein -
J7299_RS13860 (J7299_13860) 16561..16812 + 252 WP_002296238.1 hypothetical protein -
J7299_RS13865 (J7299_13865) 16827..17264 + 438 WP_002296237.1 hypothetical protein -
J7299_RS13870 (J7299_13870) 17257..17964 + 708 WP_002353391.1 hypothetical protein -
J7299_RS15715 18129..18329 + 201 WP_002330566.1 hypothetical protein -
J7299_RS13875 (J7299_13875) 18345..18524 + 180 WP_002305761.1 hypothetical protein -
J7299_RS13880 (J7299_13880) 18865..19128 + 264 WP_002287514.1 hypothetical protein Antitoxin
J7299_RS13885 (J7299_13885) 19122..19472 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J7299_RS13890 (J7299_13890) 19496..20110 - 615 WP_010729795.1 recombinase family protein -
J7299_RS13895 (J7299_13895) 20424..20846 + 423 WP_002288787.1 hypothetical protein -
J7299_RS13900 (J7299_13900) 20856..21059 + 204 WP_002299573.1 hypothetical protein -
J7299_RS13905 (J7299_13905) 21270..21854 + 585 WP_002299575.1 recombinase family protein -
J7299_RS13910 (J7299_13910) 22043..22723 - 681 WP_010861579.1 IS6-like element IS1216 family transposase -
J7299_RS13915 (J7299_13915) 22798..23475 + 678 Protein_28 PTS system mannose/fructose/sorbose family transporter subunit IID -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..235463 235463


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T198127 WP_002321032.1 NZ_CP072580:19122-19472 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T198127 NZ_CP096841:c3025806-3025390 [Mycobacterium tuberculosis variant bovis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT198127 WP_002287514.1 NZ_CP072580:18865-19128 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT198127 NZ_CP096841:c3026069-3025803 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References