Detailed information of TA system TA198123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 468758..469329
• Replicon: chromosome
• Accession: NZ_CP072579
• Organism: Enterococcus faecium strain VB12993

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J7299_RS02240
• Protein ID: WP_105468411.1
• Coordinates: 468988..469329 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: J7299_RS02235
• Protein ID: WP_002323011.1
• Coordinates: 468758..468988 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7299_RS02210 (J7299_02210)	464115..464741	+	627	WP_002352507.1	cysteine hydrolase	-
J7299_RS02215 (J7299_02215)	464924..465505	+	582	WP_002327433.1	TetR/AcrR family transcriptional regulator	-
J7299_RS02220 (J7299_02220)	465870..466445	+	576	WP_002352505.1	SOS response-associated peptidase family protein	-
J7299_RS02225 (J7299_02225)	466650..466988	-	339	WP_002306002.1	hypothetical protein	-
J7299_RS02230 (J7299_02230)	467415..468602	-	1188	WP_002296840.1	IS256-like element IS16 family transposase	-
J7299_RS02235 (J7299_02235)	468758..468988	+	231	WP_002323011.1	hypothetical protein	Antitoxin
J7299_RS02240 (J7299_02240)	468988..469329	+	342	WP_105468411.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J7299_RS02245 (J7299_02245)	470179..470364	+	186	WP_002304207.1	hypothetical protein	-
J7299_RS02250 (J7299_02250)	470869..471063	+	195	WP_002297028.1	hypothetical protein	-
J7299_RS02255 (J7299_02255)	471254..471503	+	250	Protein_444	transposase	-
J7299_RS02260 (J7299_02260)	471747..472577	-	831	WP_002286789.1	manganese catalase family protein	-
J7299_RS02265 (J7299_02265)	472785..474119	+	1335	WP_002286788.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	467415..468602	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13355.73 Da
• Isoelectric Point: 10.2170

>T198123 WP_105468411.1 NZ_CP072579:468988-469329 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVIRPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T198123 NZ_CP096841:2834412-2834825 [Mycobacterium tuberculosis variant bovis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGTCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT198123 WP_002323011.1 NZ_CP072579:468758-468988 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT198123 NZ_CP096841:2834864-2835121 [Mycobacterium tuberculosis variant bovis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9