Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 155977..157114 Replicon plasmid pVB3895_p1
Accession NZ_CP072568
Organism Enterococcus faecium strain VB3895

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag J7291_RS14225 Protein ID WP_002401483.1
Coordinates 155977..156840 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J7291_RS14230 Protein ID WP_000301765.1
Coordinates 156842..157114 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7291_RS14195 (J7291_14200) 151870..153360 - 1491 WP_002380753.1 primase C-terminal domain-containing protein -
J7291_RS14200 (J7291_14205) 153755..153994 - 240 WP_002380754.1 helix-turn-helix domain-containing protein -
J7291_RS14210 (J7291_14215) 154356..155093 - 738 WP_001038790.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
J7291_RS14215 (J7291_14220) 155218..155301 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
J7291_RS15420 (J7291_14225) 155350..155433 - 84 Protein_159 MLS leader peptide -
J7291_RS14225 (J7291_14230) 155977..156840 - 864 WP_002401483.1 zeta toxin family protein Toxin
J7291_RS14230 (J7291_14235) 156842..157114 - 273 WP_000301765.1 antitoxin Antitoxin
J7291_RS14235 (J7291_14240) 157131..157346 - 216 WP_002295735.1 peptide-binding protein -
J7291_RS14240 (J7291_14245) 157417..158313 - 897 WP_118215883.1 ParA family protein -
J7291_RS14245 (J7291_14250) 158416..158676 - 261 Protein_164 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
J7291_RS14250 (J7291_14255) 158846..159583 - 738 WP_182481801.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
J7291_RS14255 (J7291_14260) 159708..159803 - 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
J7291_RS14260 (J7291_14265) 159852..160091 - 240 WP_000635249.1 peptide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') / erm(B) - 1..210114 210114
- inside IScluster/Tn aac(6')-aph(2'') / erm(B) - 147400..163461 16061


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-213)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32486.06 Da        Isoelectric Point: 6.9965

>T198120 WP_002401483.1 NZ_CP072568:c156840-155977 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T198120 NZ_CP096841:c2381685-2381368 [Mycobacterium tuberculosis variant bovis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT198120 WP_000301765.1 NZ_CP072568:c157114-156842 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT198120 NZ_CP096841:c2381897-2381682 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References