Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 483086..483657 Replicon chromosome
Accession NZ_CP072567
Organism Enterococcus faecium strain VB3895

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag J7291_RS02305 Protein ID WP_002286801.1
Coordinates 483316..483657 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J7291_RS02300 Protein ID WP_002323011.1
Coordinates 483086..483316 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7291_RS02270 (J7291_02270) 478219..479550 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
J7291_RS02275 (J7291_02275) 479572..480198 + 627 WP_002286816.1 cysteine hydrolase -
J7291_RS02280 (J7291_02280) 480381..480962 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
J7291_RS15175 481694..482256 + 563 Protein_452 SOS response-associated peptidase family protein -
J7291_RS02295 (J7291_02295) 482461..482799 - 339 WP_002286804.1 hypothetical protein -
J7291_RS02300 (J7291_02300) 483086..483316 + 231 WP_002323011.1 hypothetical protein Antitoxin
J7291_RS02305 (J7291_02305) 483316..483657 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J7291_RS02310 (J7291_02310) 484507..484692 + 186 WP_002304207.1 hypothetical protein -
J7291_RS02315 (J7291_02315) 485197..485391 + 195 WP_002297028.1 hypothetical protein -
J7291_RS02320 (J7291_02320) 485582..485831 + 250 Protein_458 transposase -
J7291_RS02325 (J7291_02325) 486075..486905 - 831 WP_002286789.1 manganese catalase family protein -
J7291_RS02330 (J7291_02330) 487113..488447 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T198119 WP_002286801.1 NZ_CP072567:483316-483657 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T198119 NZ_CP096841:c2345022-2344588 [Mycobacterium tuberculosis variant bovis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT198119 WP_002323011.1 NZ_CP072567:483086-483316 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT198119 NZ_CP096841:c2345283-2345029 [Mycobacterium tuberculosis variant bovis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References