Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4196641..4197138 Replicon chromosome
Accession NZ_CP072566
Organism Escherichia coli strain WG5::TT12A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J8F85_RS20330 Protein ID WP_001173343.1
Coordinates 4196641..4196814 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag J8F85_RS20335 Protein ID WP_000398619.1
Coordinates 4196842..4197138 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F85_RS20305 (4191971) 4191971..4192546 + 576 WP_001188520.1 transcriptional regulator -
J8F85_RS20320 (4194214) 4194214..4194768 + 555 Protein_3986 dipeptide/tripeptide permease DtpC -
J8F85_RS20325 (4195005) 4195005..4196522 + 1518 WP_001295074.1 lysine--tRNA ligase -
J8F85_RS20330 (4196641) 4196641..4196814 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J8F85_RS20335 (4196842) 4196842..4197138 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J8F85_RS20340 (4197365) 4197365..4197637 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J8F85_RS20345 (4197649) 4197649..4197879 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
J8F85_RS20350 (4198060) 4198060..4199691 + 1632 WP_047402662.1 sensor histidine kinase -
J8F85_RS20355 (4199688) 4199688..4200407 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198117 WP_001173343.1 NZ_CP072566:c4196814-4196641 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198117 NZ_CP096841:2234567-2234965 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT198117 WP_000398619.1 NZ_CP072566:c4197138-4196842 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT198117 NZ_CP096841:2234339-2234566 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References