Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2268973..2269499 Replicon chromosome
Accession NZ_CP072566
Organism Escherichia coli strain WG5::TT12A

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag J8F85_RS10850 Protein ID WP_047402633.1
Coordinates 2269212..2269499 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag J8F85_RS10845 Protein ID WP_000534858.1
Coordinates 2268973..2269212 (+) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F85_RS10815 (2264077) 2264077..2264498 + 422 Protein_2124 hypothetical protein -
J8F85_RS10825 (2266379) 2266379..2267401 + 1023 Protein_2126 ISNCY family transposase -
J8F85_RS10830 (2267522) 2267522..2267671 + 150 WP_011443592.1 protein YdfW -
J8F85_RS10835 (2268108) 2268108..2268440 - 333 WP_001301033.1 FlxA-like family protein -
J8F85_RS10840 (2268643) 2268643..2268948 - 306 WP_001326990.1 protein YdfV -
J8F85_RS10845 (2268973) 2268973..2269212 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
J8F85_RS10850 (2269212) 2269212..2269499 + 288 WP_047402633.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
J8F85_RS10855 (2269571) 2269571..2269726 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
J8F85_RS10860 (2269943) 2269943..2270194 + 252 WP_000980994.1 protein Rem -
J8F85_RS10865 (2270261) 2270261..2270539 + 279 WP_012304870.1 hypothetical protein -
J8F85_RS10870 (2270541) 2270541..2271590 + 1050 WP_001265199.1 DUF968 domain-containing protein -
J8F85_RS10875 (2271604) 2271604..2272356 + 753 WP_001047135.1 antitermination protein -
J8F85_RS10880 (2272634) 2272634..2272723 - 90 WP_120795389.1 hypothetical protein -
J8F85_RS10885 (2272778) 2272778..2272990 - 213 WP_000087756.1 cold shock-like protein CspF -
J8F85_RS10890 (2273291) 2273291..2273506 + 216 WP_000066484.1 cold shock-like protein CspB -
J8F85_RS10895 (2273870) 2273870..2274040 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
J8F85_RS10900 (2274260) 2274260..2274475 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2256755..2290996 34241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11224.28 Da        Isoelectric Point: 10.4221

>T198109 WP_047402633.1 NZ_CP072566:2269212-2269499 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLKSPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T198109 NZ_CP096841:c1930445-1930056 [Mycobacterium tuberculosis variant bovis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT198109 WP_000534858.1 NZ_CP072566:2268973-2269212 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT198109 NZ_CP096841:c1930669-1930442 [Mycobacterium tuberculosis variant bovis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References