Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4196966..4197463 Replicon chromosome
Accession NZ_CP072565
Organism Escherichia coli strain WG5::PA8

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J8F86_RS20275 Protein ID WP_001173343.1
Coordinates 4196966..4197139 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag J8F86_RS20280 Protein ID WP_000398619.1
Coordinates 4197167..4197463 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F86_RS20250 (4192296) 4192296..4192871 + 576 WP_001188520.1 transcriptional regulator -
J8F86_RS20265 (4194539) 4194539..4195093 + 555 Protein_3975 dipeptide/tripeptide permease DtpC -
J8F86_RS20270 (4195330) 4195330..4196847 + 1518 WP_001295074.1 lysine--tRNA ligase -
J8F86_RS20275 (4196966) 4196966..4197139 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J8F86_RS20280 (4197167) 4197167..4197463 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J8F86_RS20285 (4197690) 4197690..4197962 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J8F86_RS20290 (4197974) 4197974..4198204 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
J8F86_RS20295 (4198385) 4198385..4200016 + 1632 WP_047402662.1 sensor histidine kinase -
J8F86_RS20300 (4200013) 4200013..4200732 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198094 WP_001173343.1 NZ_CP072565:c4197139-4196966 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198094 NZ_CP096841:c756230-755847 [Mycobacterium tuberculosis variant bovis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT198094 WP_000398619.1 NZ_CP072565:c4197463-4197167 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT198094 NZ_CP096841:c756480-756325 [Mycobacterium tuberculosis variant bovis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References