Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3437789..3438407 Replicon chromosome
Accession NZ_CP072565
Organism Escherichia coli strain WG5::PA8

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J8F86_RS16545 Protein ID WP_001291435.1
Coordinates 3438189..3438407 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J8F86_RS16540 Protein ID WP_000344800.1
Coordinates 3437789..3438163 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F86_RS16530 (3432878) 3432878..3434071 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J8F86_RS16535 (3434094) 3434094..3437243 + 3150 WP_081508855.1 efflux RND transporter permease AcrB -
J8F86_RS16540 (3437789) 3437789..3438163 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J8F86_RS16545 (3438189) 3438189..3438407 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J8F86_RS16550 (3438579) 3438579..3439130 + 552 WP_000102564.1 maltose O-acetyltransferase -
J8F86_RS16555 (3439246) 3439246..3439716 + 471 WP_000136192.1 YlaC family protein -
J8F86_RS16560 (3439880) 3439880..3441430 + 1551 WP_001408474.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J8F86_RS16565 (3441472) 3441472..3441825 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J8F86_RS16575 (3442204) 3442204..3442515 + 312 WP_000409911.1 MGMT family protein -
J8F86_RS16580 (3442546) 3442546..3443118 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T198089 WP_001291435.1 NZ_CP072565:3438189-3438407 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T198089 NZ_CP096841:c699906-699493 [Mycobacterium tuberculosis variant bovis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT198089 WP_000344800.1 NZ_CP072565:3437789-3438163 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT198089 NZ_CP096841:c700139-699903 [Mycobacterium tuberculosis variant bovis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References