Detailed information of TA system TA198068

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3687470..3688149
• Replicon: chromosome
• Accession: NZ_CP072564
• Organism: Escherichia coli strain WG5::PA2

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J8F87_RS17800
• Protein ID: WP_000854680.1
• Coordinates: 3687808..3688149 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J8F87_RS17795
• Protein ID: WP_000070396.1
• Coordinates: 3687470..3687787 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8F87_RS17750 (3682908)	3682908..3683729	+	822	WP_000197388.1	DUF932 domain-containing protein	-
J8F87_RS17755 (3683946)	3683946..3684647	+	702	WP_000189411.1	WYL domain-containing protein	-
J8F87_RS17760 (3684688)	3684688..3684924	+	237	WP_001144031.1	protein YpjK	-
J8F87_RS17765 (3684924)	3684924..3685367	+	444	WP_000649865.1	hypothetical protein	-
J8F87_RS17770 (3685390)	3685390..3685857	+	468	WP_001385283.1	protein YkfB	-
J8F87_RS17775 (3685934)	3685934..3686173	+	240	WP_000194654.1	DUF905 family protein	-
J8F87_RS17780 (3686271)	3686271..3686729	+	459	WP_000211838.1	antirestriction protein	-
J8F87_RS17785 (3686745)	3686745..3687221	+	477	WP_000811693.1	RadC family protein	-
J8F87_RS17790 (3687230)	3687230..3687451	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J8F87_RS17795 (3687470)	3687470..3687787	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J8F87_RS17800 (3687808)	3687808..3688149	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J8F87_RS17810 (3688721)	3688721..3689974	-	1254	WP_000893272.1	glutamate-5-semialdehyde dehydrogenase	-
J8F87_RS17815 (3689986)	3689986..3691089	-	1104	WP_001285288.1	glutamate 5-kinase	-
J8F87_RS17820 (3691377)	3691377..3692432	+	1056	WP_045145434.1	phosphoporin PhoE	-
J8F87_RS17825 (3692471)	3692471..3692872	-	402	WP_047402959.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T198068 WP_000854680.1 NZ_CP072564:3687808-3688149 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T198068 NZ_CP096840:c2838829-2838434 [Mycobacterium tuberculosis variant bovis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT198068 WP_000070396.1 NZ_CP072564:3687470-3687787 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT198068 NZ_CP096840:c2839071-2838826 [Mycobacterium tuberculosis variant bovis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7