Detailed information of TA system TA198048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1261253..1262170
• Replicon: chromosome
• Accession: NZ_CP072563
• Organism: Bacillus sp. LJBV19

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: J6600_RS06505
• Protein ID: WP_007407256.1
• Coordinates: 1261424..1262170 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: J6600_RS06500
• Protein ID: WP_003154807.1
• Coordinates: 1261253..1261423 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6600_RS06465 (J6600_06465)	1256465..1258054	+	1590	WP_209388145.1	hypothetical protein	-
J6600_RS06470 (J6600_06470)	1258067..1258492	+	426	WP_029973065.1	hypothetical protein	-
J6600_RS06475 (J6600_06475)	1258497..1258694	+	198	WP_007610833.1	XkdX family protein	-
J6600_RS06480 (J6600_06480)	1258751..1259512	+	762	WP_209388146.1	phage portal protein	-
J6600_RS06485 (J6600_06485)	1259564..1259827	+	264	WP_003154815.1	hemolysin XhlA family protein	-
J6600_RS06490 (J6600_06490)	1259841..1260104	+	264	WP_003154813.1	phage holin	-
J6600_RS06495 (J6600_06495)	1260118..1260996	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
J6600_RS19555	1261031..1261156	-	126	WP_003154809.1	hypothetical protein	-
J6600_RS06500 (J6600_06500)	1261253..1261423	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
J6600_RS06505 (J6600_06505)	1261424..1262170	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J6600_RS06510 (J6600_06510)	1262275..1263273	-	999	WP_003154805.1	inorganic phosphate transporter	-
J6600_RS06515 (J6600_06515)	1263286..1263903	-	618	WP_003154804.1	DUF47 domain-containing protein	-
J6600_RS06520 (J6600_06520)	1264190..1265506	-	1317	WP_007610842.1	amino acid permease	-
J6600_RS06525 (J6600_06525)	1265828..1266778	+	951	WP_209388147.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1227683..1320394	92711

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T198048 WP_007407256.1 NZ_CP072563:c1262170-1261424 [Bacillus sp. LJBV19]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T198048 NZ_CP096840:1538192-1539013 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT198048 WP_003154807.1 NZ_CP072563:c1261423-1261253 [Bacillus sp. LJBV19]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT198048 NZ_CP096840:1539006-1539242 [Mycobacterium tuberculosis variant bovis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14