Detailed information of TA system TA198047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 495958..496595
• Replicon: chromosome
• Accession: NZ_CP072563
• Organism: Bacillus sp. LJBV19

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: J6600_RS02475
• Protein ID: WP_003156187.1
• Coordinates: 496245..496595 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: J6600_RS02470
• Protein ID: WP_003156188.1
• Coordinates: 495958..496239 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6600_RS02450 (J6600_02450)	492323..492922	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
J6600_RS02455 (J6600_02455)	493015..493380	+	366	WP_095061578.1	holo-ACP synthase	-
J6600_RS02460 (J6600_02460)	493545..494552	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
J6600_RS02465 (J6600_02465)	494669..495838	+	1170	WP_053285486.1	alanine racemase	-
J6600_RS02470 (J6600_02470)	495958..496239	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
J6600_RS02475 (J6600_02475)	496245..496595	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
J6600_RS02480 (J6600_02480)	496713..497534	+	822	WP_003156182.1	STAS domain-containing protein	-
J6600_RS02485 (J6600_02485)	497539..497904	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
J6600_RS02490 (J6600_02490)	497907..498308	+	402	WP_003156178.1	anti-sigma regulatory factor	-
J6600_RS02495 (J6600_02495)	498320..499327	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
J6600_RS02500 (J6600_02500)	499391..499720	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
J6600_RS02505 (J6600_02505)	499717..500199	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
J6600_RS02510 (J6600_02510)	500165..500953	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
J6600_RS02515 (J6600_02515)	500953..501555	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T198047 WP_003156187.1 NZ_CP072563:496245-496595 [Bacillus sp. LJBV19]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T198047 NZ_CP096840:c1387616-1387323 [Mycobacterium tuberculosis variant bovis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT198047 WP_003156188.1 NZ_CP072563:495958-496239 [Bacillus sp. LJBV19]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT198047 NZ_CP096840:c1387882-1387613 [Mycobacterium tuberculosis variant bovis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5