Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PemK-MazE |
| Location | 17635..18170 | Replicon | plasmid pBYT1-2 |
| Accession | NZ_CP072561 | ||
| Organism | Pseudomonas sp. BYT-1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A7W6J9K5 |
| Locus tag | J5X93_RS27720 | Protein ID | WP_021561444.1 |
| Coordinates | 17635..17955 (-) | Length | 107 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A7W6J9M0 |
| Locus tag | J5X93_RS27725 | Protein ID | WP_021561443.1 |
| Coordinates | 17955..18170 (-) | Length | 72 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J5X93_RS27685 (J5X93_27555) | 13050..13808 | - | 759 | WP_011875625.1 | membrane protein | - |
| J5X93_RS27690 (J5X93_27560) | 13818..14267 | - | 450 | WP_076205818.1 | hypothetical protein | - |
| J5X93_RS27695 (J5X93_27565) | 14297..15079 | - | 783 | WP_005797951.1 | membrane protein | - |
| J5X93_RS27700 (J5X93_27570) | 15150..15746 | - | 597 | WP_011517526.1 | undecaprenyl-diphosphatase | - |
| J5X93_RS27705 (J5X93_27575) | 15803..16138 | - | 336 | WP_005797945.1 | PepSY domain-containing protein | - |
| J5X93_RS27710 (J5X93_27580) | 16459..16839 | - | 381 | WP_250036155.1 | hypothetical protein | - |
| J5X93_RS27715 (J5X93_27585) | 16836..17399 | - | 564 | WP_021561445.1 | recombinase family protein | - |
| J5X93_RS27720 (J5X93_27590) | 17635..17955 | - | 321 | WP_021561444.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| J5X93_RS27725 (J5X93_27595) | 17955..18170 | - | 216 | WP_021561443.1 | antitoxin MazE family protein | Antitoxin |
| J5X93_RS27730 (J5X93_27600) | 18459..19214 | + | 756 | WP_232476757.1 | recombinase family protein | - |
| J5X93_RS27735 (J5X93_27605) | 19216..19890 | - | 675 | WP_011178411.1 | DNA methylase | - |
| J5X93_RS27740 (J5X93_27610) | 20045..20476 | - | 432 | WP_001665065.1 | OmpA family protein | - |
| J5X93_RS27745 (J5X93_27615) | 20492..21190 | - | 699 | WP_011114053.1 | TraX family protein | - |
| J5X93_RS27750 (J5X93_27620) | 21190..21456 | - | 267 | WP_010890140.1 | hypothetical protein | - |
| J5X93_RS27755 (J5X93_27625) | 21485..22120 | - | 636 | WP_011114052.1 | transglycosylase SLT domain-containing protein | - |
| J5X93_RS27760 (J5X93_27630) | 22134..22721 | - | 588 | WP_010890138.1 | TrbM/KikA/MpfK family conjugal transfer protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..75715 | 75715 | |
| - | flank | IS/Tn | - | - | 16836..17399 | 563 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 107 a.a. Molecular weight: 11585.51 Da Isoelectric Point: 6.2890
>T198046 WP_021561444.1 NZ_CP072561:c17955-17635 [Pseudomonas sp. BYT-1]
MQRGDLVTVSLQGDYGKPRPALIVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI
MQRGDLVTVSLQGDYGKPRPALIVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI
Download Length: 321 bp
>T198046 NZ_CP096840:1383175-1383606 [Mycobacterium tuberculosis variant bovis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA
Antitoxin
Download Length: 72 a.a. Molecular weight: 8159.19 Da Isoelectric Point: 7.2801
>AT198046 WP_021561443.1 NZ_CP072561:c18170-17955 [Pseudomonas sp. BYT-1]
MPSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFTAQVRKQCQSLKGDPAEAEVLRFTEEAASHVEGWE
MPSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFTAQVRKQCQSLKGDPAEAEVLRFTEEAASHVEGWE
Download Length: 216 bp
>AT198046 NZ_CP096840:1382918-1383178 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7W6J9K5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7W6J9M0 |