Detailed information of TA system TA198043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 207422..208092
• Replicon: plasmid pKP2648-Vir
• Accession: NZ_CP072558
• Organism: Klebsiella pneumoniae strain KP2648

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: J7339_RS29210
• Protein ID: WP_004213072.1
• Coordinates: 207649..208092 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: J7339_RS29205
• Protein ID: WP_004213073.1
• Coordinates: 207422..207652 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7339_RS29180 (J7339_29080)	202607..203386	-	780	WP_004213560.1	site-specific integrase	-
J7339_RS29185 (J7339_29085)	203383..204204	-	822	WP_004213562.1	hypothetical protein	-
J7339_RS29190 (J7339_29090)	205176..205382	+	207	WP_004213077.1	hypothetical protein	-
J7339_RS29195 (J7339_29095)	205372..205665	-	294	WP_004213076.1	hypothetical protein	-
J7339_RS29200 (J7339_29100)	205681..206814	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
J7339_RS29205 (J7339_29105)	207422..207652	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J7339_RS29210 (J7339_29110)	207649..208092	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
J7339_RS29215	208241..208492	+	252	WP_186987481.1	hypothetical protein	-
J7339_RS29220 (J7339_29120)	208515..208819	-	305	Protein_227	transposase	-
J7339_RS29225 (J7339_29125)	209236..209871	+	636	Protein_228	mucoid phenotype regulator RmpA2	-
J7339_RS29230 (J7339_29130)	210389..210792	-	404	Protein_229	GAF domain-containing protein	-
J7339_RS29235 (J7339_29135)	210883..211803	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
J7339_RS29240 (J7339_29140)	211852..212343	-	492	WP_011251327.1	DM13 domain-containing protein	-
J7339_RS29245 (J7339_29145)	212406..212681	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucD / iucC / iucB / iucA / rmpA / iroN / rmpA2 / iutA	1..216902	216902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T198043 WP_004213072.1 NZ_CP072558:207649-208092 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T198043 NZ_CP096840:1165307-1166188 [Mycobacterium tuberculosis variant bovis]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT198043 WP_004213073.1 NZ_CP072558:207422-207652 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT198043 NZ_CP096840:1164687-1165310 [Mycobacterium tuberculosis variant bovis]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620