Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14833..15476 Replicon plasmid pKP2648-KPC
Accession NZ_CP072557
Organism Klebsiella pneumoniae strain KP2648

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag J7339_RS27515 Protein ID WP_001044770.1
Coordinates 15060..15476 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J7339_RS27510 Protein ID WP_001261282.1
Coordinates 14833..15063 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7339_RS27475 (10056) 10056..10835 - 780 WP_013214009.1 site-specific integrase -
J7339_RS27480 (11019) 11019..12023 - 1005 WP_011977814.1 hypothetical protein -
J7339_RS27485 (12053) 12053..12256 - 204 WP_011977813.1 hypothetical protein -
J7339_RS27490 (12302) 12302..12823 - 522 WP_013214008.1 hypothetical protein -
J7339_RS27495 (12881) 12881..13273 - 393 WP_011977811.1 hypothetical protein -
J7339_RS27500 (13310) 13310..14254 - 945 WP_011977810.1 hypothetical protein -
J7339_RS27505 (14415) 14415..14876 - 462 WP_014343465.1 hypothetical protein -
J7339_RS27510 (14833) 14833..15063 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J7339_RS27515 (15060) 15060..15476 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
J7339_RS27520 (15550) 15550..17112 + 1563 WP_004206609.1 AAA family ATPase -
J7339_RS27525 (17097) 17097..18119 + 1023 WP_000361404.1 helicase UvrD -
J7339_RS27530 (18375) 18375..19072 + 698 WP_223174630.1 IS1 family transposase -
J7339_RS27535 (19101) 19101..19373 + 273 Protein_21 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaSHV-187 / blaCTX-M-65 - 1..102746 102746


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T198036 WP_001044770.1 NZ_CP072557:15060-15476 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T198036 NZ_CP096840:716506-716913 [Mycobacterium tuberculosis variant bovis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT198036 WP_001261282.1 NZ_CP072557:14833-15063 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT198036 NZ_CP096840:716249-716509 [Mycobacterium tuberculosis variant bovis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References